Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Protein-protein Interfaces02:04

Protein-protein Interfaces

12.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K
Protein Networks02:26

Protein Networks

4.0K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.0K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

3.8K
3.8K
Synthetic Biology02:55

Synthetic Biology

4.8K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
4.8K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.6K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.6K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.2K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.2K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion.

Science (New York, N.Y.)·2026
Same author

Deep peptide recognition profiling decodes TCR specificity and enables disease-associated antigen discovery.

Nature biotechnology·2026
Same author

Predicting targeted- and immunotherapeutic response outcomes in melanoma with single-cell Raman spectroscopy and AI.

bioRxiv : the preprint server for biology·2026
Same author

Overcoming T cell tolerance to tumor self-antigens through catch-bond engineering.

Science (New York, N.Y.)·2026
Same author

Hybrid Computer Vision Model to Predict Lung Cancer in Diverse Populations.

JCO clinical cancer informatics·2026
Same author

Facile induction of immune tolerance by an interleukin-2-TGFβ surrogate agonist.

Nature·2026

Video Experimental Relacionado

Updated: Jul 21, 2025

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

7.3K

Desarrollo de la coevolución sintética y el aprendizaje automático para diseñar interacciones proteína-proteína

Aerin Yang1, Kevin M Jude1,2, Ben Lai3

  • 1Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Science (New York, N.Y.)
|July 27, 2023
PubMed
Resumen

Los científicos desarrollaron una plataforma de coevolución sintética para diseñar interacciones proteína-proteína. Este método genera diversos pares de proteínas que interactúan para aplicaciones en biotecnología y biología sintética.

Más Videos Relacionados

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

1.9K
Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
10:58

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Published on: July 25, 2013

17.1K

Videos de Experimentos Relacionados

Last Updated: Jul 21, 2025

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

7.3K
Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

1.9K
Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
10:58

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Published on: July 25, 2013

17.1K

Área de la Ciencia:

  • La bioquímica
  • Biología molecular
  • Biología sintética

Sus antecedentes:

  • Las interacciones proteína-proteína naturales evolucionan con el tiempo.
  • Recrear esta coevolución en un entorno de laboratorio es un reto.
  • Comprender el reconocimiento molecular es clave para la ingeniería de proteínas.

Objetivo del estudio:

  • Desarrollar una plataforma sintética para la coevolución proteína-proteína.
  • Para analizar el reconocimiento molecular en pares de proteínas diseñadas.
  • Para expandir la diversidad de la interfaz de proteínas utilizando métodos computacionales.

Principales métodos:

  • Creó una plataforma de coevolución proteína-proteína sintética.
  • Se han aislado pares de mutinas que interactúan entre sí de grandes bibliotecas.
  • Se ha realizado un análisis de reconocimiento molecular a nivel de sistemas.
  • Se utilizaron modelos de lenguaje de proteínas previamente entrenados para la expansión in silico.

Principales resultados:

  • Se han aislado con éxito diversos pares coevolucionados de dominio Z y afibodios.
  • Caracterizó una amplia gama de propiedades de interacción (afinidad, reactividad cruzada, ortogonalidad).
  • Capturó extensas redes de coevolución.
  • Predijo las interfaces de proteínas remodeladas computacionalmente.

Conclusiones:

  • La plataforma de coevolución sintética permite el estudio y la ingeniería de las interacciones de proteínas.
  • Este enfoque genera complejos de proteínas con propiedades de reconocimiento molecular sintonizables.
  • La integración de métodos experimentales y computacionales acelera el diseño de proteínas para la biotecnología.