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El STING humano es un canal de protones.

Bingxu Liu1,2,3, Rebecca J Carlson1,4, Ivan S Pires3

  • 1Broad Institute, Cambridge, MA, USA.

Science (New York, N.Y.)
|August 3, 2023
PubMed
Resumen

El estimulador de los genes de interferón (STING) actúa como un canal de protones, regulando los procesos celulares. Esta actividad del canal es crucial para la activación del inflamatorio y la lipidación LC3B, pero no para la inducción del interferón, que ofrece objetivos terapéuticos.

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Área de la Ciencia:

  • Inmunología
  • Biología celular
  • Biología molecular

Sus antecedentes:

  • La fuga de protones de los orgánulos señala la lipidación de la cadena ligera no canónica 3B (LC3B) y la activación del inflamatorio.
  • Estos procesos son típicamente inducidos por la activación del estimulador de los genes de interferón (STING).

Objetivo del estudio:

  • Para investigar la hipótesis de que el STING humano funciona como un canal de protones.
  • Determinar el papel de la actividad potencial del canal de STING en eventos celulares posteriores como la lipidación LC3B y la activación del inflamatorio.

Principales métodos:

  • Análisis estructural para identificar la supuesta interfaz de canal de STING.
  • Medición de los cambios de pH en el Golgi tras la activación de STING.
  • Reconstituyendo STING en liposomas para evaluar el transporte de protones.
  • Utilizando el Compuesto 53 (C53), un agonista de STING, para inhibir la actividad de STING y el flujo de protones.
  • Evaluación del impacto de C53 en la lipidación LC3B inducida por STING y la activación del inflamatorio.

Principales resultados:

  • La activación de STING condujo a un aumento del pH en el Golgi, indicando el eflujo de protones.
  • El STING reconstituido demostró capacidades de transporte de protones transmembrana en los liposomas.
  • El compuesto 53 (C53) bloqueó efectivamente el flujo de protones inducido por STING tanto en modelos celulares como en liposomas.
  • C53 también inhibió la lipidación LC3B inducida por STING y la activación del inflamatorio, destacando la necesidad de la función de canal de STING para estas vías.

Conclusiones:

  • El STING humano funciona como un canal de protones.
  • La actividad del canal de protones de STING es esencial para inducir la lipidación LC3B y la activación del inflamatorio.
  • La función de inducción de interferón de STING puede separarse de su papel en la lipidación LC3B y la activación del inflamatorio, lo que sugiere mecanismos funcionales distintos.