Jove
Visualize
Contáctanos

Videos de Conceptos Relacionados

Regulated Protein Degradation02:58

Regulated Protein Degradation

7.4K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.4K
The Proteasome02:18

The Proteasome

8.8K
Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
8.8K
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

12.3K
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA...
12.3K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

5.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
5.6K
Export of Misfolded Proteins out of the ER01:32

Export of Misfolded Proteins out of the ER

3.7K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
3.7K
The Proteasome Structure01:17

The Proteasome Structure

802
The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
802

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Substrates bind to residues lining the ring of asymmetrically engaged bacterial proteasome activator Bpa.

Nature communications·2025
Same author

Single-stranded DNA binding to the transcription factor PafBC triggers the mycobacterial DNA damage response.

Science advances·2025
Same author

A spatiotemporal map of co-receptor signaling networks underlying B cell activation.

Cell reports·2024
Same author

In situ analysis of osmolyte mechanisms of proteome thermal stabilization.

Nature chemical biology·2024
Same author

Publisher Correction: Novel WYL domain-containing transcriptional activator acts in response to genotoxic stress in rapidly growing mycobacteria.

Communications biology·2023
Same author

Novel WYL domain-containing transcriptional activator acts in response to genotoxic stress in rapidly growing mycobacteria.

Communications biology·2023
Same journal

Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

Science (New York, N.Y.)·2026
Same journal

Local signals, systemic decline.

Science (New York, N.Y.)·2026
Same journal

The mechanics of liver regeneration.

Science (New York, N.Y.)·2026
Same journal

Computing in a memory with physics.

Science (New York, N.Y.)·2026
Same journal

Retraction.

Science (New York, N.Y.)·2026
Same journal

Making time.

Science (New York, N.Y.)·2026
Ver todos los artículos relacionados
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Video Experimental Relacionado

Updated: Jul 18, 2025

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae
09:05

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Published on: April 18, 2016

29.1K

Captura de proteínas para su degradación

Charlotte M Schilling1, Eilika Weber-Ban1

  • 1Institute of Molecular Biology and Biophysics, ETH Zurich, Zurich, Switzerland.

Science (New York, N.Y.)
|August 24, 2023
PubMed
Resumen
Este resumen es generado por máquina.

Una nueva vía independiente de la ubiquitina dirige las proteínas nucleares al proteasoma para su degradación. Este hallazgo revela un nuevo mecanismo para la rotación de proteínas en el núcleo celular.

Más Videos Relacionados

Assays for the Degradation of Misfolded Proteins in Cells
10:56

Assays for the Degradation of Misfolded Proteins in Cells

Published on: August 28, 2016

12.1K
Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
10:57

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae

Published on: February 16, 2015

9.8K

Videos de Experimentos Relacionados

Last Updated: Jul 18, 2025

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae
09:05

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Published on: April 18, 2016

29.1K
Assays for the Degradation of Misfolded Proteins in Cells
10:56

Assays for the Degradation of Misfolded Proteins in Cells

Published on: August 28, 2016

12.1K
Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
10:57

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae

Published on: February 16, 2015

9.8K

Área de la Ciencia:

  • Biología celular
  • Biología molecular
  • La bioquímica

Sus antecedentes:

  • El proteosoma es una máquina celular crucial responsable de la degradación de las proteínas.
  • La degradación de las proteínas suele estar mediada por el sistema ubiquitina-proteasoma.
  • Los mecanismos para dirigir las proteínas nucleares al proteosoma no se comprenden completamente.

Objetivo del estudio:

  • Investigar una nueva vía para dirigir las proteínas nucleares al proteosoma.
  • Aclarar el papel de un mecanismo independiente de la ubiquitina en la rotación de proteínas nucleares.

Principales métodos:

  • Se utilizaron modelos de levadura para la detección genética.
  • Se emplean ensayos bioquímicos para analizar las interacciones de las proteínas.
  • Realizó microscopía para rastrear la localización de proteínas.

Principales resultados:

  • Identificó una nueva vía que apunta a proteínas nucleares específicas para la degradación proteasómica.
  • Se demostró que esta vía funciona independientemente de la conjugación de la ubiquitina.
  • Caracterización de los componentes proteicos clave involucrados en este nuevo mecanismo de orientación.

Conclusiones:

  • Existe una vía independiente de la ubiquitina para la degradación de las proteínas nucleares.
  • Esta vía representa una adición significativa a nuestra comprensión de la homeostasis de las proteínas celulares.
  • Las investigaciones adicionales sobre esta vía podrían revelar nuevos objetivos terapéuticos.