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Formación rápida de puentes de disulfuro intramoleculares utilizando luz: un método eficiente para controlar la
Feng He1, Yu Chai1, Zizhen Zeng1
1National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Chinese Medicine, Nanchang 330006, P. R. China.
Journal of the American Chemical Society
|October 3, 2023
Resumen
Los investigadores desarrollaron un método activado por luz para controlar la formación de enlaces disulfuro en péptidos. Esta estrategia utiliza un grupo de fotocaje que, tras la irradiación UV, libera tiolos que forman rápidamente enlaces disulfuro, restaurando la estructura y la función del péptido.
Área de la Ciencia:
- La bioquímica
- Química orgánica
- Biotecnología
Sus antecedentes:
- Los enlaces disulfuro son cruciales para estabilizar las estructuras secundarias de péptidos y la actividad biológica.
- El control de la formación de enlaces disulfuro es esencial para la ingeniería de péptidos y el desarrollo de fármacos.
- Los métodos existentes para la formación de enlaces disulfuro carecen de control espacio-temporal.
Objetivo del estudio:
- Introducir una nueva estrategia mediada por la luz para la formación controlada de enlaces disulfuro.
- Utilizar un grupo fotocageante como grupo protector y oxidante.
- Demostrar la restauración de la estructura y la función del péptido mediante este método.
Principales métodos:
- Utilizando el 2-nitroveratrilo (oNv) como grupo fotocageante para los tiolos.
- Irradiación de tiolos enjaulados con UV para desencadenar la formación de disulfuro.
- Evaluación de la recuperación estructural y funcional del péptido antimicrobiano taquiplesina I (IPT).
Principales resultados:
- La fotólisis de los tiolos enjaulados liberan tiolos libres y un oxidante de nitrosoareno.
- La rápida oxidación de los tiolos liberados formó enlaces disulfuro de una manera "break-to-bond".
- La restauración in situ de la estructura de la horquilla β y la actividad de la membrana de TPI se logró tras la irradiación.
Conclusiones:
- La estrategia desarrollada mediada por la luz permite un control espacial-temporal preciso de la formación de enlaces disulfuro.
- Este método restaura efectivamente la conformación del péptido y la función biológica.
- La estrategia tiene un potencial significativo para el diseño de sistemas basados en péptidos sensibles a la luz.
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