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Los receptores de glucocorticoides que carecen de actividad de unión hormonal se unen en los núcleos de las células

D B Mendel, J E Bodwell, A Munck

|December 4, 1986

Resumen

El agotamiento del trifosfato de adenosina (ATP) hace que el receptor glucocorticoide entre en un inactivo,

Área de la Ciencia:

Biología celular Biología celular.
La endocrinología molecular.
La bioquímica es la bioquímica.

Sus antecedentes:

La unión al receptor de glucocorticoides (GR) es crucial para la respuesta celular a los glucocorticoides.
El agotamiento de ATP en las células conduce a una pérdida de la capacidad de unión de GR.
Se planteó la hipótesis de la existencia de una forma inactiva de "receptor nulo" en las células empobrecidas de ATP, pero no se observó directamente.

Objetivo del estudio:

Proporcionar evidencia directa de la existencia del receptor nulo.
Para investigar la localización y abundancia del receptor nulo en las células empobrecidas de ATP.

Principales métodos:

Utilizó células de timoma de ratón WEHI-7.
Empleó un anticuerpo monoclonal específico para el receptor de glucocorticoides.
Se evaluó la localización y la unión del receptor tanto en condiciones celulares normales como en condiciones celulares empobrecidas de ATP.

Principales resultados:

El receptor nulo se detectó en los núcleos de las células empobrecidas de ATP.
La cantidad de receptor nulo en las células empobrecidas de ATP fue comparable a la cantidad de receptor en las células normales.
Esto sugiere que el receptor está presente, pero en una conformación inactiva y no vinculante.

Conclusiones:

Demuestra directamente la existencia del receptor nulo, una forma inactiva del receptor glucocorticoide.
El receptor nulo está localizado en el núcleo y está presente en cantidades significativas incluso cuando se pierde la unión hormonal.
Los hallazgos apoyan el modelo en el que los niveles de ATP regulan el estado activo de unión hormonal del receptor glucocorticoide.

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