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RPC40, un gen único para una subunidad compartida entre las polimerasas A y C de ARN de levadura

C Mann, J M Buhler, I Treich

|February 27, 1987

Resumen

El gen de la subunidad AC40 de la levadura (RPC40) es esencial para la viabilidad celular y la síntesis de las polimerasas de ARN A y C. Los mutantes muestran defectos en la producción de ARN polimerasa A y C, pero no B.

Área de la Ciencia:

Biología Molecular Biología Molecular
Genética de la levadura Genética de la levadura
Expresión génica de la expresión génica.

Sus antecedentes:

Las polimerasas de ARN de levadura A y C comparten una subunidad común, aproximadamente 40 kDa.
El gen específico que codifica esta subunidad compartida no se había identificado previamente.

Objetivo del estudio:

Para identificar, secuenciar y caracterizar el gen que codifica la subunidad compartida de 40 kDa de las polimerasas de ARN de levadura A y C.
Para investigar la función y la esencialidad de esta subunidad para la viabilidad celular y la síntesis de ARN polimerasa.

Principales métodos:

Identificación de genes, secuenciación y mutagénesis in vitro del gen de la subunidad AC40 (RPC40).
Desarrollo de un nuevo método de mezcla de plásmidos para aislar mutantes sensibles a la temperatura.
Análisis de la síntesis de ARN polimerasa en mutantes ts a temperaturas restrictivas.

Principales resultados:

El gen RPC40 es único, esencial para la viabilidad de las células de levadura y codifica una proteína de 37,6 kDa.
El promotor RPC40 contiene secuencias reguladoras que se encuentran en la ARN polimerasa C y en los genes del aparato traslacional.
Un mutante ts (ts4) demostró deterioro de la síntesis de las polimerasas de ARN A y C, pero no B, a la temperatura restrictiva.

Conclusiones:

El producto del gen RPC40 es un componente crucial requerido para la síntesis de las polimerasas A y C de ARN de levadura.
Esta subunidad juega un papel específico en la biogénesis de las ARN polimerasas A y C, distintas de la ARN polimerasa B.

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