Video Experimental Relacionado
Updated: Jun 25, 2026

10:44
Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
Published on: June 20, 2018
Una familia multigénica que codifica varias estructuras de "dedos" está presente y diferencialmente activa en los
Cell
|March 13, 1987
Resumen
Los investigadores identificaron múltiples genes de ratón similares al gen Krüppel de la mosca de la fruta, con dominios de unión al ADN. La expresión génica disminuyó significativamente durante la diferenciación celular, lo que sugiere un papel en el desarrollo.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Genética molecular La genética molecular.
- La genómica es la genómica.
Sus antecedentes:
- La Drosophila es una especie de Drosophila.
- El Krüppel es el Krüppel.
- El gen, un gen clave de control del desarrollo, tiene secuencias homólogas en el ADN genómico del ratón.
Objetivo del estudio:
- Para aislar y caracterizar los genes de ratón homólogos a los de ratón.
- El Krüppel es el Krüppel.
- y investigar su expresión durante la diferenciación celular.
Principales métodos:
- Cribado de una biblioteca genómica de ratón utilizando homología de ADN a la homología de ADN.
- El Krüppel es el Krüppel.
- sondas de codificación de dedos. sondas de codificación de dedos.
- Secuenciación de ADN y análisis de clones aislados.
- Estudiar la expresión génica en células de teratocarcinoma F9 diferenciadas con ácido retinoico y cAMP.
Principales resultados:
- Se aislaron seis fagos homólogos; se secuenciaron dos clones con estructuras similares a dedos.
- La expresión de ambos genes se redujo significativamente en la diferenciación celular F9.
- Se encontraron genes homólogos con estructuras putativas de dedos en otros ADN eucariotas.
Conclusiones:
- El ADN genómico del ratón contiene múltiples copias de genes homólogos.
- El Krüppel es el Krüppel.
- , con dominios de los dedos que se unen al ácido nucleico.
- Estos genes pertenecen a una familia multigénica en los organismos superiores.
- Su expresión está regulada durante la diferenciación celular, lo que implica un papel en los procesos de desarrollo.
Videos de Conceptos Relacionados
From DNA to Protein
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
Gene Families
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Evolutionary Relationships through Genome Comparisons
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
Multi-species Conserved Sequences
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Gene Duplication and Divergence
The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are characterized.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are characterized.
Gene Families
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...

