Video Experimental Relacionado
Updated: May 11, 2026
11:07
Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
Published on: September 21, 2011
Colocalización de las funciones de unión al ADN y de activación transcripcional en el receptor humano de
Cell
|April 10, 1987
Resumen
Los investigadores identificaron un dominio central en el receptor glucocorticoide humano (hGR) responsable de la unión al ADN y la activación transcripcional. Este dominio es este dominio.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética La genética.
- La bioquímica es la bioquímica.
Sus antecedentes:
- El receptor glucocorticoide humano (hGR) es un regulador clave de la expresión génica.
- Estudios anteriores indicaron que la hGR comprende dominios funcionales discretos.
Objetivo del estudio:
- Investigar las funciones funcionales de los dominios específicos de hGR en la activación transcripcional.
- Para identificar el dominio mínimo requerido para la unión y transactivación del ADN.
Principales métodos:
- Prueba de expresión transitoria.
- Mutagénesis in vitro. mutagénesis en el laboratorio.
- Prueba de transcripción génica de fusión MTV-CAT. ensayo de transcripción génica. ensayo de fusión MTV-CAT. ensayo de transcripción génica. ensayo de fusión MTV-CAT. ensayo de transcripción génica. ensayo de fusión MTV-CAT. ensayo de fusión MTV-CAT. ensayo de transcripción génica. ensayo de fusión MTV-CAT.
Principales resultados:
- La eliminación de la mitad amino-terminal o el dominio inmunogénico reduce parcialmente la activación transcripcional.
- La eliminación del dominio de unión a los esteroides dio lugar a un receptor constitutivamente activo.
- Se identificó una región central rica en cisteína (88 aminoácidos) como esencial tanto para la unión al ADN como para la trans-activación.
Conclusiones:
- El dominio de unión de esteroides normalmente reprime la actividad de la hGR.
- Un dominio central que contiene funciones de unión y transactivación del ADN fue delineado en hGR.
- Esta función vinculada distingue a la hGR de otras proteínas reguladoras eucariotas.
Videos de Conceptos Relacionados
02:13Cooperative Binding of Transcription Regulators
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
02:42Eukaryotic Transcription Activators
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
01:30General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:13Cooperative Binding of Transcription Regulators
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...