Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Nuclear Export01:42

Nuclear Export

3.6K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
3.6K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

7.7K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.7K
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

4.6K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
4.6K
Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

3.2K
Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
3.2K
Regulated mRNA Transport02:22

Regulated mRNA Transport

6.3K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.3K
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

5.7K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
5.7K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

A phospholipid Camptothecin-Niraparib conjugate self-assembled into supramolecular nanotubes for combination cancer therapy.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

RPS5 modulates inflammatory response and angiogenesis in coronary heart disease through NF-κB signaling activation.

Molecular and cellular biochemistry·2026
Same author

Dynamic changes and relationships among AI Literacy, job crafting, and career growth in new nurses in the AI era: A multicenter three-wave longitudinal study.

Nurse education today·2026
Same author

Quantitative comparison of methodologies for translation site imaging in living cells.

RNA (New York, N.Y.)·2026
Same author

Elevated desmoglein-2 expression in multiple myeloma is a prognostic marker across genomic subtypes with impact on high-risk cytogenetics and a distinct gene expression profile.

British journal of haematology·2026
Same author

Integrative pan-cancer analysis identifies CCHCR1 as a prognostic biomarker and therapeutic target driving EMT in hepatocellular carcinoma via PI3K/AKT activation.

Genes & diseases·2026

Video Experimental Relacionado

Updated: Jul 3, 2025

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.2K

Exportación nuclear de ARN circular

Linh H Ngo1, Andrew G Bert2, B Kate Dredge2,3,4

  • 1RNA Biology and Cancer Laboratory, Peter MacCallum Cancer Centre and Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia.

Nature
|February 14, 2024
PubMed
Resumen

Los investigadores descubrieron una nueva vía de exportación nuclear para los ARN circulares (ARN circulares). Esta vía utiliza la exportina-2 y el IGF2BP1, regulados por Ran-GTP, ofreciendo nuevos conocimientos sobre la regulación del circRNA.

Más Videos Relacionados

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.3K
In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

1.5K

Videos de Experimentos Relacionados

Last Updated: Jul 3, 2025

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.2K
Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.3K
In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

1.5K

Área de la Ciencia:

  • Biología molecular
  • Biología celular
  • Biología del ARN

Sus antecedentes:

  • Los ARN circulares (circRNA) se forman por el empalme del ARNm precursor.
  • Los circRNA juegan un papel en las células normales y cancerosas.
  • Los circRNA son principalmente citoplasmáticos, lo que requiere la exportación nuclear.

Objetivo del estudio:

  • Identificar la vía específica para la exportación nuclear de ARN circulares.
  • Para aclarar los mecanismos moleculares que rigen la exportación nuclear de circRNA.

Principales métodos:

  • Se investigó el papel de Ran-GTP, exportin-2 y IGF2BP1 en la exportación de circRNA.
  • Manipulado el gradiente nuclear Ran-GTP usando la inhibición y el agotamiento de CRM1.
  • Se ha realizado el nocaut/agotamiento de la exportina-2.
  • Se analizaron las proteínas de unión al circRNA nuclear y sus interacciones.

Principales resultados:

  • Se identificó una nueva vía que requiere Ran-GTP, exportin-2 e IGF2BP1 para la exportación nuclear de circRNA.
  • La modulación del gradiente nuclear Ran-GTP afectó las tasas de exportación de circRNA.
  • El agotamiento de la exportina-2 inhibió específicamente la exportación nuclear del circRNA.
  • Ran-GTP mejora la interacción entre el IGF2BP1 y los circARN.

Conclusiones:

  • Una vía dependiente de Ran-GTP que incluye exportin-2 e IGF2BP1 facilita la exportación nuclear del circRNA.
  • Este mecanismo es análogo a la exportación de proteínas, distinto de la exportación de ARNm.
  • Las proteínas adaptadoras como IGF2BP1 son cruciales para reclutar maquinaria de exportación para los circRNA.