Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

G-protein Coupled Receptors01:21

G-protein Coupled Receptors

91.4K
G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
91.4K
GPCR Desensitization01:12

GPCR Desensitization

6.1K
G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
6.1K
GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

6.9K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
6.9K
Transducer Mechanism: G Protein–Coupled Receptors01:30

Transducer Mechanism: G Protein–Coupled Receptors

9.0K
G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical,...
9.0K
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

4.4K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
4.4K
Microbial Biosensors01:17

Microbial Biosensors

88
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
88

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Divergent Roles of mGlu2 and mGlu3 Receptors in Amyloid-β Production and Cognitive Dysfunctions in Alzheimer's Disease.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Inhibitory probes for spatiotemporal analysis of Gα<sub>s</sub> protein signaling.

Nature chemical biology·2026
Same author

A sensitive biosensor of endogenous Gα<sub>i</sub> activity enables the accurate characterization of endogenous GPCR agonist responses.

Science signaling·2025
Same author

Direct detection of endogenous Gαi activity in cells with a sensitive conformational biosensor.

bioRxiv : the preprint server for biology·2024
Same author

TrkB receptor interacts with mGlu<sub>2</sub> receptor and mediates antipsychotic-like effects of mGlu<sub>2</sub> receptor activation in the mouse.

Science advances·2024
Same author

Direct interrogation of context-dependent GPCR activity with a universal biosensor platform.

bioRxiv : the preprint server for biology·2024

Video Experimental Relacionado

Updated: May 1, 2026

Monitoring GPCR-&#946;-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery
08:21

Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery

Published on: June 28, 2019

6.9K

Interrogación directa de la actividad GPCR dependiente del contexto con una plataforma de biosensores universal

Remi Janicot1, Marcin Maziarz1, Jong-Chan Park1

  • 1Department of Biochemistry & Cell Biology, Chobanian & Avedisian School of Medicine, Boston University, Boston, MA 02118, USA.

Cell
|February 27, 2024
PubMed
Resumen

Los investigadores desarrollaron una nueva plataforma de biosensores para medir la actividad del receptor acoplado a proteínas G (GPCR) en las células. Esta herramienta ofrece información de alta fidelidad sobre la señalización GPCR, ayudando al descubrimiento de fármacos y a comprender las respuestas celulares.

Palabras clave:
El proyecto BRETProteínas GGPCR y sus derivadosLa GTPasaLos antipsicóticosseñalización sesgadaBiosensor y sus componentesdescubrimiento de fármacosFibrosis en el cuerpoTransmisor neurológico

Más Videos Relacionados

Parallel Interrogation of &#946;-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay
09:03

Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay

Published on: March 10, 2020

12.3K
A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors
08:46

A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors

Published on: December 2, 2022

2.2K

Videos de Experimentos Relacionados

Last Updated: May 1, 2026

Monitoring GPCR-&#946;-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery
08:21

Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery

Published on: June 28, 2019

6.9K
Parallel Interrogation of &#946;-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay
09:03

Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay

Published on: March 10, 2020

12.3K
A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors
08:46

A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors

Published on: December 2, 2022

2.2K

Área de la Ciencia:

  • La bioquímica
  • Biología molecular
  • Farmacología

Sus antecedentes:

  • Los receptores acoplados a proteínas G (GPCR) son una clase importante de objetivos farmacológicos.
  • Las herramientas actuales carecen de la precisión para estudiar las GPCR en entornos fisiológicos.
  • Comprender el comportamiento de la GPCR es crucial para el desarrollo de nuevas terapias.

Objetivo del estudio:

  • Crear una plataforma escalable para la medición de la actividad GPCR.
  • Permitir una evaluación de alta fidelidad de la activación de la proteína G por cualquier GPCR.
  • Investigar la señalización GPCR en las células endógenas y primarias.

Principales métodos:

  • Desarrollo de construcciones compactas de biosensores ONE vector G-protein Optical (ONE-GO).
  • Caracterización de numerosos GPCR en diversos tipos de células, incluidas las células cardiovasculares primarias y las neuronas.
  • Aplicación de la plataforma para el estudio de los GPCR en condiciones relacionadas con la enfermedad.

Principales resultados:

  • Se reveló información sobre la base molecular de la selectividad de acoplamiento de GPCR a la proteína G.
  • Determinación de los perfiles farmacogenómicos de los antipsicóticos en las variantes naturales del GPCR.
  • Se ha identificado un sesgo de señalización del subtipo de proteína G específico para el tipo de célula y dependiente del estado de la enfermedad mediante GPCR endógenos.

Conclusiones:

  • La plataforma de código abierto ONE-GO proporciona una amplia accesibilidad para el estudio de la actividad GPCR dependiente del contexto.
  • Esta herramienta facilita una comprensión más profunda de la función de GPCR en la salud y la enfermedad.
  • Permite la interrogación directa de la señalización GPCR en sistemas fisiológicamente relevantes.