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La diversidad subestimada de las modificaciones de los ácidos biliares
Ipsita Mohanty1, Helena Mannochio-Russo1, Joshua V Schweer2
1Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA, USA.
Cell
|March 12, 2024
Resumen
Los investigadores descubrieron numerosas modificaciones de ácido biliar previamente desconocidas utilizando una nueva biblioteca espectral de EM / EM. Estas modificaciones, incluidos los amidatos biliares de poliamina, se encuentran en humanos y cambian con la dieta, lo que afecta a la investigación de la salud y las enfermedades.
Área de la Ciencia:
- Metabolomía
- La bioquímica
- Metabolismo microbiano
Sus antecedentes:
- Las modificaciones del ácido biliar por el huésped y el metabolismo microbiano no se comprenden completamente.
- Una comprensión completa de estas modificaciones es crucial para estudiar su papel en la salud y la enfermedad.
Objetivo del estudio:
- Crear un recurso para la identificación de nuevas modificaciones de ácido biliar.
- Investigar la prevalencia y el impacto dietético de las modificaciones específicas del ácido biliar en los seres humanos.
Principales métodos:
- Se filtraron 1.200 millones de espectros de masas en tándem (MS/MS) para patrones de iones selectivos de ácido biliar.
- Desarrolló una biblioteca espectral de ácido biliar MS / MS centrada en la modificación.
- Utilizó la biblioteca para identificar nuevos metabolitos, incluidos los amidatos biliares de poliamina.
Principales resultados:
- Identificó miles de modificaciones de ácido biliar no reconocidas previamente en especies y cultivos microbianos.
- Los amidatos biliares de poliamina descubiertos son prevalentes en los carnívoros y están presentes en los humanos.
- Se han observado alteraciones en los niveles de amidato biliar poliamínico en humanos tras un cambio de dieta.
Conclusiones:
- Hay significativamente más modificaciones de ácido biliar de lo que se conocía anteriormente.
- Los datos metabolómicos disponibles públicamente pueden aprovecharse para descubrir nuevos metabolitos.
- La biblioteca de EM/EM desarrollada permitirá avanzar en la investigación sobre las funciones de los ácidos biliares en la salud y las enfermedades.
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