Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

13.2K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.2K
RNA Stability01:53

RNA Stability

33.5K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.5K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

7.7K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.7K
Types of RNA01:20

Types of RNA

5.8K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
5.8K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

11.5K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.5K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Impact of repeated auditing in antimicrobial stewardship programs on prescribing appropriateness: a ten-year observational cohort study of a national surveillance program.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2026
Same author

Looking Back to Move Forward: A Narrative Review of Indigenous Health Intervention Research by the University Departments of Rural Health Against a Contemporary National Framework.

International journal of environmental research and public health·2026
Same author

Implementation of the BIOFIRE Meningitis/Encephalitis Panel: A Mixed-Methods Implementation Study in a Nonmetropolitan Tertiary Hospital.

Open forum infectious diseases·2026
Same author

Contributions of Australian University Departments of Rural Health to Indigenous Health Intervention Research: A Narrative Review.

Healthcare (Basel, Switzerland)·2026
Same author

Impact of iron overload on hematopoietic stem cell transplantation in pediatric patients with nonmalignant hematological disorders.

Blood advances·2026
Same author

Consumer Perspectives on PICU Safety and Quality.

Nursing in critical care·2026

Video Experimental Relacionado

Updated: Jun 29, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

25.3K

ARN nucleares excepcionalmente de larga duración

Jeanne Lawrence1,2, Lisa Hall1,2

  • 1Department of Neurology, University of Massachusetts Chan Medical School, Worcester, MA, USA.

Science (New York, N.Y.)
|April 4, 2024
PubMed
Resumen
Este resumen es generado por máquina.

El ARN recién sintetizado en ratones jóvenes persiste en el cerebro durante al menos dos años. Este hallazgo revela la estabilidad a largo plazo del ARN y su papel potencial en la función cerebral adulta.

Más Videos Relacionados

Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells
06:29

Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

Published on: April 7, 2023

4.9K
Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

23.6K

Videos de Experimentos Relacionados

Last Updated: Jun 29, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

25.3K
Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells
06:29

Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

Published on: April 7, 2023

4.9K
Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

23.6K

Área de la Ciencia:

  • La neurociencia
  • Biología molecular
  • La genética

Sus antecedentes:

  • Las moléculas de ARN juegan un papel crucial en la expresión génica y la función celular.
  • La vida útil y la persistencia del ARN dentro del cerebro no se comprenden completamente.
  • Investigar la estabilidad del ARN es clave para comprender los procesos neuronales a largo plazo.

Objetivo del estudio:

  • Para determinar la persistencia a largo plazo del ARN recién sintetizado en el cerebro del ratón.
  • Investigar la estabilidad de las moléculas de ARN durante un período prolongado.

Principales métodos:

  • El ARN recién sintetizado se etiquetó en ratones jóvenes utilizando una técnica de etiquetado específica.
  • Se recogió tejido cerebral de estos ratones en varios momentos, incluidos 2 años después del etiquetado.
  • Se emplearon métodos de detección y cuantificación de ARN para evaluar la presencia de ARN.

Principales resultados:

  • El ARN marcado se detectó con éxito en los cerebros de ratones adultos 2 años después del etiquetado inicial.
  • La presencia de ARN persistente sugiere una estabilidad significativa dentro del entorno neural.

Conclusiones:

  • El ARN recién sintetizado puede permanecer estable en el cerebro del ratón durante al menos 2 años.
  • Esta persistencia de ARN a largo plazo puede tener implicaciones para la memoria, el aprendizaje y otras funciones cerebrales adultas.