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Descubrimiento y caracterización de las 5'-fosfato dependientes de piridoxal sintasas de cicloleucina

Abner N D Abad¹, Kaushik Seshadri¹, Masao Ohashi¹

¹Departments of Chemical and Biomolecular Engineering, University of California, Los Angeles, California 90095, United States.

Journal of the American Chemical Society

|May 14, 2024

Resumen

Las enzimas dependientes del piridoxal 5'-fosfato (PLP) catalizan las nuevas anulaciones [3 + 2] para sintetizar aminoácidos cuaternarios como la cicloleucina. Estos biocatalizadores permiten reacciones en tándem, ampliando su utilidad en la síntesis de bloques de construcción farmacéuticos.

Área de la Ciencia:

La bioquímica
Enzimología
Síntesis orgánica

Sus antecedentes:

Las enzimas dependientes del piridoxal 5-fosfato (PLP) son biocatalizadores cruciales para la síntesis de aminoácidos no proteígenos.
Los aminoácidos cuaternarios, como la cicloleucina, son bloques de construcción farmacéuticos valiosos.

Objetivo del estudio:

Descubrir y caracterizar nuevas enzimas dependientes de PLP involucradas en la biosíntesis de cicloleucina.
Para aclarar el mecanismo de la formación de enlaces de carbono-carbono en tándem que conduce a la anulación [3 + 2].

Principales métodos:

Descubrimiento y caracterización de enzimas.
Ensayos bioquímicos para estudiar la actividad de las enzimas y los mecanismos de reacción.
Análisis de los productos de reacción.

Principales resultados:

Descubrimiento de las sintasas de cicloleucina, una familia de enzimas dependientes de PLP.
Demostración de la formación de enlaces C-C en tándem a través de la sustitución γ seguida de reacciones intramoleculares de aldol o de Mannich.
Catálisis de la anulación global [3 + 2] para producir derivados de 2-hidroxi o 2-aminocicloeleucina.

Conclusiones:

Las sintasas de cicloleucina amplían el repertorio biocatalítico de las enzimas dependientes de PLP.
Estas enzimas permiten una síntesis eficiente de aminoácidos cuaternarios complejos.
Los hallazgos ofrecen nuevas vías para la síntesis farmacéutica utilizando biocatálisis.

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