Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Thermosensation01:43

Thermosensation

30.4K
Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
30.4K
Increased Body Temperature01:25

Increased Body Temperature

664
A body temperature above  38°C  (100.4 °F) is known as fever or pyrexia, and a person with fever is termed 'febrile.' Typically, the hypothalamus, a part of the brain that acts as the body's thermostat, regulates body temperature through a thermoregulatory setpoint. It receives signals from cold and warm thermal receptors throughout the body and adjusts the body's temperature accordingly. Fever occurs when this hypothalamic setpoint is altered, usually in...
664
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

2.2K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
2.2K
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

6.4K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
6.4K
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

4.6K
GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...
4.6K
Body Temperature01:25

Body Temperature

941
The body's temperature, measured in degrees, is determined by the balance between heat production and dissipation to the surrounding environment. For instance, if exercising vigorously, the body will produce more heat, causing sweat and dissipating that heat. Despite extreme environmental conditions and physical exertion, the human temperature-control system maintains a constant core body temperature (the temperature of deep tissues, which are the tissues located beneath the skin and other...
941

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Temperature and intrinsic Ca<sup>2+</sup> reshape TRPM4 pharmacology.

Nature structural & molecular biology·2026
Same author

Tumor cells-derived CXCL17 is associated with up-regulated neutrophil to monocyte ratio and predicts poor prognosis in oral squamous cell carcinoma.

Frontiers in oncology·2026
Same author

Identification and classification of ion channels across the tree of life provide functional insights into understudied CALHM channels.

eLife·2026
Same author

A R2R3-MYB, VwMYB1, regulates anthocyanin biosynthesis in pansy petal blotch via transcriptional activation of VwF3'5'H.

Plant cell reports·2026
Same author

Structural Insights and Multi-center Luminescence in Cr<sup>3+</sup>-Doped Gallogermanate for High-Efficiency Near-Infrared pc-LEDs.

ACS applied materials & interfaces·2026
Same author

Application of CAR-T therapy in solid tumors: current opportunities and challenges.

Cancer gene therapy·2026

Video Experimental Relacionado

Updated: Jun 26, 2025

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
08:35

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

Published on: March 17, 2015

14.8K

Dispositivos fisiológicos para el reconocimiento del ligando TRPM4 y el gating

Jinhong Hu1, Sung Jin Park1, Tyler Walter1,2

  • 1Van Andel Institute, Grand Rapids, MI, USA.

Nature
|May 15, 2024
PubMed
Resumen

El estudio del canal iónico TRPM4 a temperaturas fisiológicas revela un

Más Videos Relacionados

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
12:09

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

10.1K
Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
11:53

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

8.0K

Videos de Experimentos Relacionados

Last Updated: Jun 26, 2025

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
08:35

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

Published on: March 17, 2015

14.8K
Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
12:09

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

10.1K
Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
11:53

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

8.0K

Área de la Ciencia:

  • La biofísica
  • Biología estructural
  • Fisiología de los canales iónicos

Sus antecedentes:

  • La función macromolecular, especialmente la actividad de las proteínas, está significativamente influenciada por la temperatura.
  • Los estudios biofísicos a menudo descuidan las temperaturas fisiológicas, lo que potencialmente produce ideas biológicas inexactas.
  • Los canales iónicos sensibles a la temperatura, como el TRPM4, juegan un papel crucial en los procesos fisiológicos.

Objetivo del estudio:

  • Investigar las características estructurales y funcionales dependientes de la temperatura del canal iónico TRPM4.
  • Comprender cómo funciona el TRPM4 e interactúa con los ligandos a temperaturas fisiológicas.
  • Elucidar el mecanismo de bloqueo de TRPM4 bajo condiciones térmicas fisiológicamente relevantes.

Principales métodos:

  • Microscopía crioelectrónica de una sola partícula (cryo-EM) de TRPM4 a temperaturas fisiológicas.
  • Análisis estructural para identificar las conformaciones distintas.
  • Ensayos funcionales para evaluar la unión de ligandos y la apertura de canales.

Principales resultados:

  • Se identificó una nueva conformación "caliente" de TRPM4 a temperaturas fisiológicas, que difiere de las estructuras a baja temperatura.
  • Se encontró que un sitio de unión de calcio dependiente de la temperatura en el dominio intracelular era crucial para la función de TRPM4.
  • Se demostró que los sitios de unión de ligandos para el decavanadato y el ATP son dependientes de la temperatura, con relevancia funcional.
  • Se aclaró el mecanismo de entrada de TRPM4, revelando la apertura del canal a temperaturas fisiológicas, que no se había observado anteriormente.

Conclusiones:

  • TRPM4 exhibe propiedades estructurales y funcionales distintas a temperaturas fisiológicas.
  • El estudio de las macromoléculas, particularmente los canales iónicos, a temperaturas fisiológicas es crítico para una comprensión mecánica y farmacológica precisa.
  • Los hallazgos proporcionan un marco molecular para la comprensión de la percepción de la temperatura del canal TRPM termo-sensible.