Video Experimental Relacionado
Updated: Jun 25, 2025
06:15
Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion
Published on: August 15, 2016
7.7K
Interfaces adhesivas anti-fibróticas en diversos órganos
Jingjing Wu1, Jue Deng1, Georgios Theocharidis2
1Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Nature
|May 22, 2024
Resumen
Una interfaz adhesiva implante-tejido evita la formación de cápsulas fibrosas alrededor de los dispositivos médicos. Este nuevo enfoque reduce la inflamación y mantiene la función del dispositivo, ofreciendo una estrategia prometedora para las interfaces anti-fibróticas a largo plazo.
Área de la Ciencia:
- Ciencia de los biomateriales
- Ingeniería de tejidos
- Desarrollo de dispositivos médicos
Sus antecedentes:
- Los biomateriales implantados a menudo fallan a largo plazo debido a las reacciones de cuerpos extraños y la formación de cápsulas fibrosas en la interfaz implante-tejido.
- Esta encapsulación fibrótica compromete la funcionalidad y eficacia del dispositivo.
- El desarrollo de estrategias para mitigar esta reacción es crucial para mejorar el rendimiento de los dispositivos implantables.
Objetivo del estudio:
- Investigar el potencial de una interfaz adhesiva implante-tejido para mitigar la formación de cápsulas fibrosas.
- Evaluar el impacto de esta interfaz en la infiltración de las células inflamatorias.
- Evaluar la biocompatibilidad y la funcionalidad a largo plazo de los productos con interfaz adhesiva.
Principales métodos:
- Se utilizaron diversos modelos animales (ratas, ratones, ratones humanizados, cerdos) para probar las interfaces adhesivas.
- Se realizó un análisis histológico para evaluar la formación de cápsulas fibrosas y la infiltración de células inflamatorias.
- Se han realizado ensayos in vitro (adsorción de proteínas, Luminex, qPCR, inmunofluorescencia, secuenciación de ARN) para la validación mecanicista.
- Se ha evaluado la comunicación eléctrica bidireccional a largo plazo de electrodos implantables con interfaces adhesivas en ratas.
Principales resultados:
- Las interfaces implante- tejido adhesivas redujeron significativamente la infiltración de células inflamatorias en comparación con las interfaces no adhesivas.
- No se observaron cápsulas fibrosas en varios órganos durante 12 semanas in vivo.
- Se ha demostrado una comunicación eléctrica bidireccional sostenida a través de electrodos implantables con interfaces adhesivas durante 12 semanas.
- Los análisis in vitro han validado la hipótesis anti-fibrótica.
Conclusiones:
- Una interfaz adhesiva implante-tejido mitiga efectivamente la formación de cápsulas fibrosas y la inflamación.
- Esta estrategia apoya la biocompatibilidad a largo plazo y la funcionalidad del producto.
- Las interfaces adhesivas representan un enfoque prometedor para el desarrollo de dispositivos médicos implantables avanzados y duraderos.
Videos de Conceptos Relacionados
01:21Layers of Connective Tissue Proper
1.9K
Fascia, a thin layer of fibrous connective tissue, is distributed throughout the body. It demarcates and forms a supportive covering over skeletal muscles, bones, blood vessels, and organs. There are three main types of facia— superficial fascia, deep fascia, and subserous fascia. These are all present at different depths in the body. Fascia reduces the friction and permits muscles, joints, and organs to easily slide against each other, facilitating movement of the body and preventing...
1.9K
01:25Fibronectins Connect Cells with ECM
2.4K
Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
2.4K
01:24Adherens Junctions
4.7K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
4.7K
01:17Intracellular Signaling Affects Focal Adhesions
2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.7K
01:27Tissue Membranes
6.5K
A tissue membrane is a thin layer of cells that covers the outside of the body, the organs, internal passageways that lead to the exterior of the body, and the lining of the moveable joint cavities. There are two basic types of tissue membranes— connective tissue and epithelial membranes.
Connective Tissue Membranes
The connective tissue membrane is formed solely from connective tissue. These membranes encapsulate organs, such as the kidneys, and line our movable joints. A synovial...
Connective Tissue Membranes
The connective tissue membrane is formed solely from connective tissue. These membranes encapsulate organs, such as the kidneys, and line our movable joints. A synovial...
6.5K
01:24Overview of Cell-Matrix Interactions
7.2K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
7.2K