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FilamentID revela la composición y función de los polímeros de las enzimas metabólicas durante la gametogénesis

Jannik Hugener¹, Jingwei Xu², Rahel Wettstein³

¹Institute of Molecular Biology and Biophysics, ETH Zürich, 8093 Zürich, Switzerland; Institute of Biochemistry, ETH Zürich, 8093 Zürich, Switzerland; Max Perutz Labs, University of Vienna, 1030 Vienna, Austria.

|June 21, 2024

Resumen

Las células forman filamentos únicos durante la latencia. Estas estructuras, identificadas utilizando FilamentID, ayudan a las esporas de levadura a recuperarse y volver a entrar en el ciclo celular después de la inanición.

Área de la Ciencia:

Biología celular
Biología estructural
Genética de la levadura

Sus antecedentes:

La latencia celular es crucial para la reproducción, pero los mecanismos de recuperación no están claros.
Las células de levadura en ciernes muestran un desarrollo suspendido durante la formación de gametos y el desarrollo de esporas.

Objetivo del estudio:

Para investigar las bases estructurales de la recuperación celular de la latencia en la levadura en ciernes.
Identificar la composición molecular y la función de los conjuntos filamentosos observados durante la meiosis y el desarrollo de las esporas.

Principales métodos:

Tomografía cryoelectrónica (cryoET) y microscopía cryoelectrónica (cryoEM) para imágenes de alta resolución.
Un nuevo flujo de trabajo de "identificación de filamentos" (FilamentID) que combina imágenes y análisis a múltiples escalas.
La perturbación genética para estudiar la formación de filamentos y la recuperación celular.

Principales resultados:

Descubrió conjuntos filamentosos en el núcleo, el citoplasma y las mitocondrias de las células de levadura.
Filamentos mitocondriales identificados como compuestos por la aldehído deshidrogenasa Ald4 (ALDH2).
Los filamentos nucleoplásmicos/citoplásmicos identificados como acetil-CoA sintetasa Acs1 (ACSS2), revelan los mecanismos de polimerización.
Se demostró que la polimerización de Acs1 ayuda a la recuperación de las esporas envejecidas y a la reentrada del ciclo celular en las células hambrientas.

Conclusiones:

FilamentID es una herramienta poderosa para caracterizar filamentos celulares desconocidos.
La polimerización de Acs1 juega un papel clave en la recuperación celular de la latencia y el hambre.
La comprensión de estas estructuras de filamentos proporciona información sobre las estrategias fundamentales de supervivencia celular.

Palabras clave:

el estrés celular Estructura in situ Biología estructural integradora el metabolismo desarrollo de métodos Imágenes en múltiples escalas Filamentos de proteínas Quiescencia el hambre

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