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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

piRNA - Piwi-interacting RNAs

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Las partículas de RNP en las secuencias de unión de empalme en las transcripciones del corión de Drosophila.

Y N Osheim, O L Miller, A L Beyer

|November 1, 1985

Resumen

Las partículas específicas de ribonucleoproteína (RNP) se unen a las transcripciones de ARN nacientes en las uniones de empalme. Estas partículas RNP pueden desempeñar un papel crucial en el empalme de ARN al mantener los sitios de empalme juntos durante el procesamiento.

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Área de la Ciencia:

Biología Molecular Biología Molecular
Genética La genética.
La bioquímica es la bioquímica.

Sus antecedentes:

Las partículas heterogéneas de ribonucleoproteína nuclear (HnRNP) se observan en las transcripciones de ARN nacientes.
La importancia funcional de los sitios de unión de partículas de HnRNP en el ARN aún no se ha aclarado completamente.

Objetivo del estudio:

Investigar el papel de secuencias específicas unidas por partículas HnRNP en el procesamiento de ARN.
Para correlacionar la morfología de la transcripción naciente con las secuencias de nucleótidos en los genes del corión de Drosophila.

Principales métodos:

Visualización microscópica de electrones de la cromatina extendida.
Correlación de la morfología de la transcripción naciente con las secuencias de nucleótidos de los genes Drosophila chorion s36-1 y s38-1.

Principales resultados:

Las partículas HnRNP (aprox. 25 nm) se localizan en las uniones de empalme de intrones en las transcripciones de coriones nacientes.
Una partícula más grande (aprox. 40 nm), posiblemente formado por coalescencia de partículas, se observa en transcripciones más maduras cerca de las uniones de empalme.

Conclusiones:

Las estructuras RNP observadas en las uniones de empalme sugieren un papel en el empalme de ARN.
Estas partículas RNP pueden facilitar la unión y el mantenimiento de las uniones de empalme durante el empalme bipartito.