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Dos polipéptidos Lyt-2 surgen de un solo gen mediante patrones de empalme alternativos de ARNm
Cell
|November 1, 1985
Resumen
La molécula Lyt-2, un marcador de linfocitos T, está codificada por un solo gen. El empalme de ARNm alternativo genera dos cadenas polipeptídicas Lyt-2 que difieren en la longitud de la cola citoplasmática.
Área de la Ciencia:
- Inmunología Inmunología.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- La molécula Lyt-2/3 es una glicoproteína de linfocitos T.
- Se considera un marcador para las células T citotóxicas / supresoras.
- Se postula que es un receptor para las proteínas MHC clase I.
Objetivo del estudio:
- Para identificar el homólogo de ratón de la proteína humana Leu-2/T8.
- Para investigar la base genética de la diversidad de la cadena de polipéptido Lyt-2.
- Para dilucidar los mecanismos moleculares que generan las variantes Lyt-2.
Principales métodos:
- Aislamiento de ADNc de ratón y clones genómicos utilizando una sonda de ADNc humana Leu-2/T8.
- Experimentos de transfección para confirmar la homología genética.
- Secuenciación de nucleótidos de clones de ADNc que codifican las variantes Lyt-2.
Principales resultados:
- El homólogo en ratón de Leu-2/T8 fue identificado como Lyt-2, no Lyt-3.
- Un solo gen codifica dos cadenas de polipéptidos Lyt-2.
- El empalme alternativo de ARNm conduce a variaciones en la longitud de la cola citoplasmática.
Conclusiones:
- La molécula Lyt-2 se genera a partir de un solo gen mediante el empalme de ARNm alternativo.
- Las diferencias observadas en las cadenas de polipéptido Lyt-2 se deben a las diferentes longitudes de la cola citoplasmática.
- Este estudio aclara la base genética y molecular de la expresión de Lyt-2 en linfocitos T.
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