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Published on: February 28, 2019
Influencia del polimorfismo alélico en el ensamblaje y la expresión superficial de moléculas MHC (Ia) de clase II
Cell
|November 1, 1985
Resumen
La expresión génica del complejo mayor de histocompatibilidad (MHC) alfa y beta murina clase II en células L reveló que las parejas de haplotipos no coincidentes tenían una baja expresión de Ia en la superficie celular. Variación alélica en el gen A beta del gen.
Área de la Ciencia:
- Inmunogenética La inmunogenética.
- Biología molecular La biología molecular.
Sus antecedentes:
- La expresión de Ia en la superficie celular es crucial para las respuestas inmunes.
- Las moléculas del complejo mayor de histocompatibilidad (MHC) clase II presentan antígenos a las células T.
Objetivo del estudio:
- Investigar el impacto de la variación alélica en los genes MHC murinos alfa y beta de clase II en la expresión de la superficie celular Ia.
- Para identificar el control genético del ensamblaje y la expresión de Ia.
Principales métodos:
- Transfección de los genes MHC alfa y beta murinos de clase II en células L.
- Análisis de la expresión de Ia en la superficie celular utilizando citometría de flujo.
- Análisis de ARN para cuantificar los niveles de transcripción de los genes MHC.
Principales resultados:
- Los pares de genes coincidentes con el haplotipo resultaron en una alta expresión de Ia, mientras que los pares no coincidentes con el haplotipo mostraron una expresión inesperadamente baja.
- Se requirieron niveles elevados de transcripción A beta y A alfa en transfectantes no coincidentes para niveles similares de membrana Ia.
- El control del ensamblaje/expresión de Ia por una variación alelica beta fue asignado al dominio NH2-terminal (beta 1).
Conclusiones:
- La variación alélica en el gen A beta influye significativamente en el ensamblaje y la expresión del MHC clase II.
- Estos hallazgos pueden explicar el desequilibrio de enlace observado en los genes de la región MHC I.
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