Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.9K
Gene Conversion02:08

Gene Conversion

9.6K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
9.6K
Homologous Recombination02:31

Homologous Recombination

50.1K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.1K
Crossing Over01:30

Crossing Over

4.1K
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
4.1K
Viral Recombination00:57

Viral Recombination

23.2K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
23.2K
Exon Recombination02:32

Exon Recombination

3.5K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
3.5K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Towards the construction of a virtual yeast.

Nature·2026
Same author

Harmonizing standards and resources for the medical genome.

Nature·2026
Same author

PerturbPlan: An analytical framework for designing Perturb-seq experiments.

bioRxiv : the preprint server for biology·2026
Same author

Targeted single-cell RNA and perturbation sequencing with TAP-seq.

Nature protocols·2026
Same author

CAMK2D causes heart failure in mice with RBM20 cardiomyopathy.

Nature cardiovascular research·2026
Same author

Genome-scale mapping of variant, enhancer and gene function in primary human CD4+ T cells.

bioRxiv : the preprint server for biology·2026
Same journal

A native sulfur deposit in Gale crater, Mars.

Science (New York, N.Y.)·2026
Same journal

Coordinated demise of harmful algal blooms.

Science (New York, N.Y.)·2026
Same journal

Genetic effects put into context.

Science (New York, N.Y.)·2026
Same journal

Bacteria share proteins to survive antibiotics.

Science (New York, N.Y.)·2026
Same journal

Impacts shaped Earth's first continents.

Science (New York, N.Y.)·2026
Same journal

Erratum for the Report "Covalently bonded single-molecule junctions with stable and reversible photoswitched conductivity" by C. Jia <i>et al</i>.

Science (New York, N.Y.)·2026
Ver todos los artículos relacionados

Video Experimental Relacionado

Updated: May 30, 2025

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

16.5K

Recombinación del genoma bajo demanda

Marta Seczynska1, Lars M Steinmetz1,2,3

  • 1Department of Genetics, School of Medicine, Stanford University, Stanford, CA, USA.

Science (New York, N.Y.)
|January 30, 2025
PubMed
Resumen
Este resumen es generado por máquina.

Los grandes reordenamientos del genoma ahora se pueden crear de manera eficiente en las células de los mamíferos. Este avance permite estudios genómicos a gran escala y aplicaciones en varios campos biológicos.

Más Videos Relacionados

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
11:40

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

12.0K
Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

19.2K

Videos de Experimentos Relacionados

Last Updated: May 30, 2025

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

16.5K
Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
11:40

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

12.0K
Recombineering Homologous Recombination Constructs in Drosophila
14:23

Recombineering Homologous Recombination Constructs in Drosophila

Published on: July 13, 2013

19.2K

Área de la Ciencia:

  • La genómica
  • Biología celular
  • Biología molecular

Sus antecedentes:

  • Los reordenamientos del genoma son cruciales para la función celular y la evolución.
  • Los métodos anteriores para generar grandes reordenamientos del genoma eran limitados en escala y eficiencia.

Objetivo del estudio:

  • Desarrollar un método escalable para generar grandes reordenamientos del genoma en células de mamíferos.
  • Para permitir estudios de alto rendimiento de la inestabilidad del genoma y la reparación del ADN.

Principales métodos:

  • Utilizó la tecnología de edición de genes CRISPR-Cas9.
  • Los ARN guía específicos diseñados para inducir rupturas de doble cadena en las ubicaciones genómicas deseadas.
  • Se emplean vías de reparación del ADN para facilitar los reordenamientos.

Principales resultados:

  • Generó con éxito una variedad de grandes reordenamientos del genoma, incluidas deleciones, inversiones y translocaciones.
  • Escalabilidad demostrada del método en diferentes tipos de células de mamíferos.
  • Validación de la exactitud y eficiencia de los reordenamientos generados.

Conclusiones:

  • El método desarrollado proporciona una herramienta poderosa para la ingeniería genómica a gran escala en células de mamíferos.
  • Este avance acelerará la investigación en áreas como la genómica del cáncer, la biología del desarrollo y la biología sintética.