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Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
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Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high affinity and are together...
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Updated: Jun 14, 2026

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
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Interacción ultraespecífica G-cuadruplex-colistina para el mapeo eficiente de todo el transcriptoma G4

Shijiong Wei1, Xiaobo Zhang1, Yilong Feng2

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Journal of the American Chemical Society
|March 6, 2025
PubMed
Resumen

El antibiótico colistina (COL) se une específicamente a los cuadruplexos G paralelos (G4), haciendo que se agreguen. Este descubrimiento permitió el desarrollo de CoRP-seq, un método simple para mapear el ARN G4 en células humanas.

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Área de la Ciencia:

  • Biología Química
  • La genómica
  • Biología molecular

Sus antecedentes:

  • Los G-cuadruplexos (G4s) son estructuras complejas de ácido nucleico con polimorfismo dinámico, que plantean desafíos para las intervenciones de biología química.
  • Dirigirse a los G4 es crucial para comprender los procesos celulares y desarrollar nuevas terapias.

Objetivo del estudio:

  • Investigar la interacción de pequeñas moléculas con estructuras específicas de G4.
  • Desarrollar un nuevo método para evaluar la prevalencia de ARN G-cuadruplexos en el transcriptoma humano.

Principales métodos:

  • Se explotó la interacción específica entre el antibiótico colistina (COL) y los cuadruplexos G paralelos (G4).
  • Desarrolló el protocolo de precipitación y secuenciación de ARN G4 inducido por COL (CoRP-seq), utilizando agregación y centrifugación específicas de la estructura para el aislamiento de G4.
  • Se aplicó CoRP-seq para evaluar la prevalencia de ARN G4 en los transcriptomas de células humanas.

Principales resultados:

  • La colistina (COL) se une selectivamente a las G-cuadruplexas paralelas (G4).
  • Esta interacción específica induce la agregación del complejo G4/COL, lo que permite la separación por centrifugación.
  • El protocolo CoRP-seq demostró una ultraespecificidad, simplicidad y conveniencia para el mapeo de ARN G4.

Conclusiones:

  • El descubrimiento de la interacción específica G4 de COL proporciona una nueva herramienta para la orientación G4.
  • CoRP-seq ofrece un método sencillo y altamente específico para el perfil de ARN G-cuadruplex.
  • Este avance aborda las necesidades no satisfechas en el mapeo G4 y el campo más amplio de la G4ómica.