Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.0K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.0K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

23.1K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
23.1K
Transcription Initiation01:47

Transcription Initiation

16.1K
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
16.1K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

9.0K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.0K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

28.2K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
28.2K
The Replisome03:01

The Replisome

32.8K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
32.8K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

MBD8 acts as a conserved cofactor of the histone demethylase LDL2 to regulate flowering time in plants.

Journal of integrative plant biology·2026
Same author

Mechanism and reconstitution of circadian transcription in cyanobacteria.

Nature structural & molecular biology·2026
Same author

Repair of damaged lysosomes by TECPR1-mediated membrane tubulation during energy crisis.

Cell research·2026
Same author

A homolog of methionine γ-lyase is required for biofilm development in the cyanobacterium Synechococcus elongatus.

World journal of microbiology & biotechnology·2025
Same author

Robert Haselkorn (1934-2025): Pioneer in molecular biology and microbiology.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Nutrient-driven TOR signalling controls a chromatin-associated complex for orchestrating plant growth and stress tolerance.

Nature plants·2025

Video Experimental Relacionado

Updated: May 20, 2025

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
07:42

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Published on: September 17, 2016

12.7K

El bucle P de la NTPasa RUVBL2 es un componente de reloj conservado en los eucariotas

Meimei Liao1, Yanqin Liu1,2, Zhancong Xu1,3

  • 1National Institute of Biological Sciences, Beijing, China.

Nature
|March 27, 2025
PubMed
Resumen
Este resumen es generado por máquina.

El reloj circadiano eucariota utiliza la enzima RUVBL2, que tiene una actividad ATPasa notablemente lenta. Este hallazgo revela RUVBL2 como un componente conservado en todas las especies, lo que sugiere que la hidrólisis lenta de ATP es una característica compartida de los relojes biológicos.

Más Videos Relacionados

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
10:38

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

22.3K
In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
11:56

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Published on: September 28, 2017

9.7K

Videos de Experimentos Relacionados

Last Updated: May 20, 2025

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
07:42

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Published on: September 17, 2016

12.7K
Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
10:38

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

22.3K
In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
11:56

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Published on: September 28, 2017

9.7K

Área de la Ciencia:

  • Cronología
  • Biología molecular
  • La bioquímica

Sus antecedentes:

  • Los relojes circadianos eucariotas comparten una arquitectura conservada, pero carecen de un ancestro molecular común.
  • Se sabe que la enzima RUVBL2 influye en la fase circadiana y la amplitud en los relojes de los mamíferos.

Objetivo del estudio:

  • Para investigar el papel de RUVBL2 en el reloj circadiano eucariota.
  • Determinar el mecanismo por el cual RUVBL2 influye en los ritmos circadianos.

Principales métodos:

  • Evaluación de las variantes de RUVBL2 para los efectos sobre los ritmos circadianos de la actividad locomotora en ratones.
  • Ensayos enzimáticos para medir la actividad de la ATPasa del tipo salvaje RUVBL2.
  • Análisis de las interacciones físicas entre los ortólogos RUVBL2 y las proteínas del reloj del núcleo entre especies.

Principales resultados:

  • RUVBL2 influye en el período circadiano a través de su actividad ATPasa excepcionalmente lenta (hidrolizando ~ 13 moléculas de ATP / día).
  • Los mutantes de RUVBL2 exhibieron ritmos circadianos alterados, incluidos fenotipos arritmicos, de corto período y de largo período.
  • Los ortólogos de RUVBL2 interactúan con las proteínas del reloj central en humanos, Drosophila y Neurospora, mostrando una función de reloj conservada.

Conclusiones:

  • RUVBL2 se establece como un componente central común de los relojes circadianos eucariotas.
  • La actividad lenta de la ATPasa, previamente observada en las cianobacterias, es una característica compartida de los relojes eucariotas.
  • Los hallazgos sugieren un mecanismo conservado para el tiempo a través de diversas formas de vida.