Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

The Z-Scheme of Electron Transport in Photosynthesis01:34

The Z-Scheme of Electron Transport in Photosynthesis

9.7K
The light reactions of photosynthesis assume a linear flow of electrons from water to NADP+. During this process, light energy drives the splitting of water molecules to produce oxygen. However, oxidation of water molecules is a thermodynamically unfavorable reaction and requires a strong oxidizing agent. This is accomplished by the first product of light reactions: oxidized P680 (or P680+), the most powerful oxidizing agent known in biology. The oxidized P680 that acquires an electron from the...
9.7K
Photochemical Electrocyclic Reactions: Stereochemistry01:26

Photochemical Electrocyclic Reactions: Stereochemistry

1.8K
The absorption of UV–visible light by conjugated systems causes the promotion of an electron from the ground state to the excited state. Consequently, photochemical electrocyclic reactions proceed via the excited-state HOMO rather than the ground-state HOMO. Since the ground- and excited-state HOMOs have different symmetries, the stereochemical outcome of electrocyclic reactions depends on the mode of activation; i.e., thermal or photochemical.
Selection Rules: Photochemical Activation
1.8K
The Photochemical Reaction Center01:29

The Photochemical Reaction Center

4.0K
Reaction centers are pigment-protein complexes that initiate energy conversion from photons to chemical entities. Therefore, photochemical reaction center is a more appropriate term that describes these complexes. The Nobel laureates Robert Emerson and William Arnold provided the first experimental evidence of photochemical reaction centers by demonstrating the participation of nearly 2,500 chlorophyll molecules for the release of just one molecule of oxygen. Despite thousands of photosynthetic...
4.0K
Thermal and Photochemical Electrocyclic Reactions: Overview01:26

Thermal and Photochemical Electrocyclic Reactions: Overview

2.3K
Electrocyclic reactions are reversible reactions. They involve an intramolecular cyclization or ring-opening of a conjugated polyene. Shown below are two examples of electrocyclic reactions. In the first reaction, the formation of the cyclic product is favored. In contrast, in the second reaction, ring-opening is favored due to the high ring strain associated with cyclobutene formation.
2.3K
Cycloaddition Reactions: MO Requirements for Photochemical Activation01:12

Cycloaddition Reactions: MO Requirements for Photochemical Activation

2.0K
Some cycloaddition reactions are activated by heat, while others are initiated by light. For example, a [2 + 2] cycloaddition between two ethylene molecules occurs only in the presence of light. It is photochemically allowed but thermally forbidden.
2.0K
Sharpless Epoxidation02:57

Sharpless Epoxidation

3.7K
The conversion of allylic alcohols into epoxides using the chiral catalyst was discovered by K. Barry Sharpless and is known as Sharpless epoxidation. The use of a chiral catalyst enables the formation of one enantiomer of the product in excess. This chiral catalyst is mainly a chiral complex of titanium tetraisopropoxide and tartrate ester (specific stereoisomer). The stereoisomer used in the chiral catalyst dictates the formation of the enantiomer of the product. In other words, the use of...
3.7K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Inhibition of ferroptosis via SLC25A39-NRF2 axis drives Osimertinib resistance in lung adenocarcinoma.

Cellular and molecular life sciences : CMLS·2026
Same author

Fully-connected microwave photonic multi-beamformer with fast beam-steering for broadband wireless communication.

Nature communications·2026
Same author

TELO2-interacting protein 1 (TTI1), a novel Wnt/β-catenin target gene, decreases chemo-sensitivity in colorectal cancer by modulating DNA damage responses.

Molecular biomedicine·2026
Same author

Constructing a biomimetic ECM protective barrier: a strategic interface design for urethral repair to mitigate foreign body reaction.

Journal of nanobiotechnology·2026
Same author

Janus Calix[6]Arene Dual-Site Additive Enables Dendrite-Free Aqueous Zn Batteries.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Challenges in Diagnosing High-grade B-cell Lymphoma, NOS: Poor Interobserver Agreement on Its Morphologic Definition-An LLMPP Study.

The American journal of surgical pathology·2026

Video Experimental Relacionado

Updated: May 14, 2025

Light-driven Enzymatic Decarboxylation
09:58

Light-driven Enzymatic Decarboxylation

Published on: May 22, 2016

10.6K

Deracimización impulsada por la luz mediante una fotoenzima diseñada

Min Li1, Yan Zhang2, Kai Fu1

  • 1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, P. R. China.

Journal of the American Chemical Society
|April 12, 2025
PubMed
Resumen
Este resumen es generado por máquina.

Los investigadores diseñaron una nueva fotoenzima artificial utilizando la expansión del código genético para lograr la desacimización catalítica del ciclopropano. Este avance permite una nueva biocatálisis para transformaciones químicas desafiantes.

Más Videos Relacionados

Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions
10:21

Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions

Published on: October 5, 2019

8.3K
[DPEPhosbcpCu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst
09:12

[DPEPhosbcpCu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst

Published on: May 21, 2019

9.1K

Videos de Experimentos Relacionados

Last Updated: May 14, 2025

Light-driven Enzymatic Decarboxylation
09:58

Light-driven Enzymatic Decarboxylation

Published on: May 22, 2016

10.6K
Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions
10:21

Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions

Published on: October 5, 2019

8.3K
[DPEPhosbcpCu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst
09:12

[DPEPhosbcpCu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst

Published on: May 21, 2019

9.1K

Área de la Ciencia:

  • Biocatálisis
  • Ingeniería de proteínas
  • Biología sintética

Sus antecedentes:

  • Las enzimas con habilidades abiológicas ofrecen nuevas rutas biocatalíticas.
  • El biocatálisis tradicional lucha con reacciones termodinámicamente desfavorables como la desacimización del ciclopropano.

Objetivo del estudio:

  • Reutilizar un nuevo andamio de proteínas (CTB10) como una fotoenzima artificial.
  • Para permitir la desacemiación catalítica del ciclopropano mediante el uso de la biocatálisis de ingeniería.

Principales métodos:

  • La expansión del código genético se usó para crear la fotoenzima artificial.
  • La evolución dirigida se empleó para la optimización estructural.
  • Se utilizó la cristalografía de rayos X para determinar la estructura del complejo enzima- sustrato.

Principales resultados:

  • La fotoenzima diseñada basada en CTB10 logró la desacemiación catalítica del ciclopropano.
  • Se obtuvieron un amplio alcance de sustrato y altas enantioselectividades después de la optimización.
  • El análisis estructural reveló una cavidad quiral esculpida que facilita la reacción.

Conclusiones:

  • Este estudio demuestra el potencial de las fotoenzimas diseñadas para desafiar las reacciones de desacimización.
  • La fotoenzima artificial desarrollada amplía el alcance de la biocatálisis más allá de las enzimas naturales.