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La ingeniería de los comportamientos multicelulares de la levadura a través de la adhesión sintética y la

Fankang Meng¹, William M Shaw², Yui Kei Keith Kam²

¹Imperial Centre for Engineering Biology, Imperial College London, London SW7 2AZ, UK; Department of Bioengineering, Imperial College London, London SW7 2AZ, UK.

|July 12, 2025

Resumen

Los investigadores diseñaron la levadura Saccharomyces cerevisiae para la multicelularidad utilizando nuevos conjuntos de herramientas para la comunicación y la adhesión celular. Estos sistemas permiten comportamientos complejos como circuitos lógicos y ensayos de interacción de proteínas, expandiendo las aplicaciones biotecnológicas.

Área de la Ciencia:

Biología sintética
Ingeniería celular
Biotecnología

Sus antecedentes:

La coordinación multicelular es clave para la complejidad biológica.
La levadura Saccharomyces cerevisiae tiene capacidades multicelulares naturales limitadas.
La ingeniería multicelular en la levadura ofrece un potencial biotecnológico significativo.

Objetivo del estudio:

Desarrollar conjuntos de herramientas modulares para la ingeniería de comportamientos multicelulares en levaduras.
Para permitir la señalización dependiente del contacto y la adhesión celular específica.
Crear herramientas para sistemas multicelulares complejos y ensayos de interacción proteína-proteína.

Principales métodos:

Desarrollo del MARS (sistema de respuesta anclado al péptido de apareamiento) para la señalización dependiente del contacto.
Desarrollo del Saccharomyces SATURN (juego de herramientas de adhesión para el patrón multicelular) para la agregación celular.
Integración de MARS y SATURN para construir circuitos lógicos multicelulares y el sensor JUPITER.

Principales resultados:

MARS permite la comunicación yuxtacrina a través de péptidos y receptores exhibidos en la superficie.
SATURN permite patrones de agregación celular programables utilizando proteínas de adhesión.
Los sistemas combinados facilitan los circuitos lógicos multicelulares y el sensor JUPITER para las interacciones proteína-proteína y la selección de nanobodies.

Conclusiones:

Los conjuntos de herramientas desarrollados proporcionan bloques de construcción versátiles para sistemas de levadura multicelulares complejos y definidos por el usuario.
Estos avances amplían el alcance de Saccharomyces cerevisiae para aplicaciones biotecnológicas.
Los sistemas diseñados imitan los programas de desarrollo, permitiendo la diferenciación celular basada en la densidad de población local.

Palabras clave:

La señalización GPCR Adhesión entre células las comunicaciones célula-célula La señalización yuxtacrina Interacciones proteína-proteína Biología sintética conjunto de herramientas de biología sintética Multicelularidad sintética ingeniería de levaduras

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