Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

220
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
220
CRISPR01:59

CRISPR

52.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.9K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.1K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.1K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.4K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Optical-Controlled One-Pot RPA-CRISPR Assay for Environmental DNA Detection of a Critically Endangered Species.

ACS sensors·2026
Same author

Analysis of the factors influencing reoperation for severe finger scar contracture in children-clinical data analysis of 61 children.

Frontiers in pediatrics·2026
Same author

Preoperative fatty infiltration predicts functional outcomes after arthroscopic rotator cuff repair: does trauma or degeneration matter?

BMC musculoskeletal disorders·2026
Same author

Silk fibroin/nano-hydroxyapatite scaffolds with graphene oxide induce macrophage M2 polarization and enhance angiogenesis to facilitate bone repair.

RSC advances·2026
Same author

A temporal causal deep learning for real-time traffic risk prediction on freeway merging areas.

Accident; analysis and prevention·2026
Same author

A Biomimetic Copper-Caffeic Acid Nanozyme Activates Cuproptosis and Pyroptosis by Mimicking the Neutrophil Enzymatic Cascade.

Advanced healthcare materials·2026

Video Experimental Relacionado

Updated: Sep 11, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.4K

Cascada enzimática fotocontrolada programable para el diagnóstico robusto de CRISPR

Menglu Hu1, Yihui Wang1, Weiwei Qi1

  • 1School of Life Sciences, South China Normal University, Guangzhou 510631, China.

Journal of the American Chemical Society
|August 13, 2025
PubMed
Resumen

Este estudio introduce un nuevo sistema de diagnóstico CRISPR fotocontrolado para la detección de ácido nucleico. La tecnología supera las limitaciones del motivo adyacente del protospacer y permite la detección simultánea de genes duales, mejorando las capacidades de diagnóstico.

Más Videos Relacionados

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.8K
A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.2K

Videos de Experimentos Relacionados

Last Updated: Sep 11, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.4K
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.8K
A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

4.2K

Área de la Ciencia:

  • Biotecnología
  • Diagnóstico molecular
  • Tecnología CRISPR

Sus antecedentes:

  • Los diagnósticos CRISPR-Cas12a ofrecen una detección avanzada de ácido nucleico, pero se enfrentan a limitaciones.
  • Los requisitos del motivo adyacente del protospacer (PAM, por sus siglas en inglés) restringen la selección del sitio objetivo.
  • Las capacidades limitadas de multiplexación impiden la detección simultánea de múltiples objetivos.

Objetivo del estudio:

  • Para desarrollar un sistema de diagnóstico CRISPR con fotocontrol.
  • Para superar las limitaciones de PAM y mejorar la multiplexación en el diagnóstico CRISPR.
  • Permitir la detección simultánea de genes objetivo y controles internos para mejorar la utilidad clínica.

Principales métodos:

  • Se empleó una estrategia de cascada enzimática fotocontrolada.
  • Las reacciones secuenciales incluyeron amplificación de ácido nucleico, generación de ssDNA a través de la exonucleasa lambda y detección de Cas12a independiente de PAM.
  • La escisión ortogonal de Cas12a y Cas13a facilitó la detección de genes duales.

Principales resultados:

  • El sistema logró con éxito la detección independiente de PAM.
  • Se demostró la detección simultánea de genes duales utilizando Cas12a y Cas13a.
  • Se detectaron con precisión muestras clínicas de Mycobacterium tuberculosis (MTB) junto con un gen de control interno (ACTB).

Conclusiones:

  • La tecnología de diagnóstico CRISPR de un solo bote con fotocontrol aumenta la flexibilidad y supera las limitaciones de los métodos convencionales.
  • Este enfoque avanza en la aplicación clínica de diagnósticos basados en CRISPR al permitir la detección simultánea de genes objetivo y de control.
  • El sistema desarrollado muestra una promesa significativa para mejorar el diagnóstico molecular.