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La expresión génica diferencial impulsa la transición dependiente del ciclo celular de crecimiento monopolar a
Samridhi Pathak1, Maitreyi Das1
1Department of Biology, Boston College, 140 Chestnut Hill Ave, Chestnut Hill, MA 02467.
bioRxiv : the preprint server for biology
|August 20, 2025
Resumen
La progresión del ciclo celular, no solo el crecimiento, es clave para que las células de levadura cambien de un solo extremo a un crecimiento de dos extremos. Las vías de respuesta al estrés, como la respuesta a las feromonas, inhiben esta transición.
Área de la Ciencia:
- Biología celular
- Biología molecular
- La genética
Sus antecedentes:
- La polaridad celular es crucial para la estructura y función celular.
- En S. pombe, las células pasan del crecimiento monopolar al bipolar a medida que maduran.
- La fase del ciclo celular, no sólo el crecimiento, influye en esta transición.
Objetivo del estudio:
- Investigar el papel de la fase del ciclo celular en la transición del crecimiento monopolar al bipolar en S. pombe.
- Identificar genes y vías expresadas diferencialmente durante las fases G1/S y G2 que regulan esta transición.
Principales métodos:
- Secuenciación de ARNm de alto rendimiento de *cdc10-129* mutante del ciclo celular en las fases G1/S y G2.
- Análisis diferencial de la expresión génica mediante el paquete DESeq2 R.
- Análisis de la red de interacción entre proteínas.
Principales resultados:
- Se identificaron 65 genes regulados al alza en la fase G1/S (respuesta a las feromonas MAPK, plegamiento de proteínas, procesamiento de ARNr, unión a proteínas por choque térmico).
- Se identificaron 35 genes regulados en la fase G2 (mantenimiento de la membrana plasmática, organización de la pared celular).
- Se identificaron núcleos genéticos clave: *cdc15-hob3-rho1-bgs1* en G2 y *spk1-byr2-ste11* en G1/S, con Spk1 previniendo el crecimiento bipolar prematuro.
Conclusiones:
- La vía de respuesta de feromonas, regulada por Spk1, inhibe el crecimiento bipolar y promueve la detención de G1.
- La desregulación de las vías de respuesta al estrés y la progresión del ciclo celular permiten la transición al crecimiento bipolar en condiciones ricas en nutrientes.
Palabras clave:
Cdc42 (en inglés)Vía KEGGMAPK (en inglés)La polarizaciónciclo celularSecuenciación del ARNmMás Videos Relacionados
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