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Updated: Sep 10, 2025
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La actividad de la fosfatasa mediada por el módulo de fosforilación CgDbf2/CgMob1-CgCdc14 regula la señalización de
Jiyun Yang1, Mei-Ling Sun1, Yu-Ting Pan1
1Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.
Plant, cell & environment
|August 21, 2025
Resumen
La vía de salida mitótica fúngica (MEN), que involucra a CgDbf2 / CgMob1 y CgCdc14, es crucial para la infección de las plantas al coordinar la integridad de la pared celular y las respuestas de las especies reactivas de oxígeno.
Área de la Ciencia:
- Micología
- Patología molecular de las plantas
- Biología celular
Sus antecedentes:
- La vía de salida mitótica (MEN) regula la morfología celular y la patogenicidad en eucariotas.
- Los mecanismos que vinculan la señalización MEN, la integridad de la pared celular (CWI), la homeostasis de las especies reactivas de oxígeno (ROS) del huésped y la virulencia en los hongos fitopatógenos se comprenden mal.
Objetivo del estudio:
- Esclarecer el papel de la vía MEN, específicamente CgDbf2 y CgMob1, en la virulencia del hongo fitopatógeno Colletotrichum gloeosporioides.
- Investigar la interacción entre CgDbf2, CgMob1 y la fosfatasa CgCdc14 en la regulación de la patogenicidad fúngica.
Principales métodos:
- Secuenciación de ARN (RNA-seq)
- Análisis bioquímicos
- Análisis genéticos
- Identificación del complejo proteico
- Mapeo del sitio de fosforilación
Principales resultados:
- CgDbf2 y CgMob1 forman un complejo crítico en C. gloeosporioides, esencial para la integridad de la pared celular (CWI) y la resistencia a los fungicidas.
- La alteración de CgDBF2 o CgMOB1 afecta gravemente la virulencia debido a defectos en la formación del apressorio y el crecimiento invasivo.
- CgDbf2 fosforila CgCdc14 en Ser427 y Thr428, aumentando su actividad fosfatasa y contribuyendo a la virulencia.
- El eje CgDbf2/CgMob1-CgCdc14 integra las respuestas CWI y ROS, vitales para la infección de las plantas.
Conclusiones:
- El complejo CgDbf2/CgMob1 es esencial para la virulencia de C. gloeosporioides, mediando la CWI y la resistencia a los fungicidas.
- La fosforilación de CgCdc14 por CgDbf2 es crítica para la patogenicidad fúngica, destacando un cruce entre MEN y CWI.
- Este estudio proporciona nuevos conocimientos sobre los mecanismos de virulencia de los hongos y los posibles objetivos antifúngicos.
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