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Los analíticos bioquímicos en muestras de sangre centrifugadas podrían verse afectados por la edad de los sujetos en
Mahsa Yazdanbakhsh1, Mohammad Reza Deyhim2, Hanieh Jafary1
1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Asian journal of transfusion science
|August 21, 2025
Resumen
La preparación de muestras de sangre para los ancianos y los jóvenes difiere. Los individuos mayores mostraron niveles más altos de glucosa y urea, mientras que los individuos más jóvenes tenían niveles más altos de magnesio y óxido nítrico, lo que indica diferencias en la estabilidad de la muestra relacionadas con la edad.
Área de la Ciencia:
- La bioquímica clínica
- Gerontología
- Medicina de laboratorio
Sus antecedentes:
- Los protocolos estándar de preparación de muestras de sangre se aplican universalmente, independientemente de la edad del paciente.
- La sangre de las personas mayores puede ser más susceptible a las condiciones de preparación que la sangre de las personas más jóvenes.
Objetivo del estudio:
- Investigar el impacto de la duración y la temperatura de almacenamiento en los analitos bioquímicos en muestras de suero de participantes ancianos y jóvenes.
- Determinar si la edad influye en la estabilidad de las muestras de sangre en diversas condiciones de almacenamiento.
Principales métodos:
- Se recogieron muestras de sangre de 30 participantes sanos (15 jóvenes, 15 ancianos).
- Muestras de suero almacenadas en diferentes intervalos de tiempo (2h, 24h) y temperaturas (4°C, 25°C, -20°C).
- Se analizaron los analíticos bioquímicos utilizando un analizador automatizado, kits comerciales y reactivo Griess.
Principales resultados:
- Los niveles de lactato deshidrogenasa mostraron cambios significativos en los participantes jóvenes y mayores después de 2h y 24h de incubación a 20-24°C y 4°C.
- Los participantes de edad avanzada tenían niveles de glucosa y urea más altos al inicio y durante las 24 horas en comparación con los participantes más jóvenes.
- Los participantes más jóvenes mostraron niveles de magnesio y óxido nítrico más altos al inicio y durante las 24 horas que los participantes mayores.
Conclusiones:
- La edad afecta significativamente la estabilidad de ciertos analitos bioquímicos en muestras de sangre.
- La duración del almacenamiento y la temperatura afectan los niveles del analito de manera diferente en los individuos de edad avanzada frente a los jóvenes.
- Los protocolos de preparación de muestras de sangre específicos de la edad pueden ser necesarios para un diagnóstico de laboratorio preciso.
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