Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Targeted Cancer Therapies02:57

Targeted Cancer Therapies

7.8K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.8K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

17.0K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.0K
Combination Therapies and Personalized Medicine02:50

Combination Therapies and Personalized Medicine

5.1K
Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
5.1K
Mutagenicity and Carcinogenicity01:25

Mutagenicity and Carcinogenicity

1.4K
Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...
1.4K
Combined Effects of Drugs: Synergism01:27

Combined Effects of Drugs: Synergism

4.7K
Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
4.7K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Highly Specific Inhibition of <i>c-Myc</i> Oncogene Expression through Shifting the G-Quadruplex toward a Triplex Structure.

Journal of medicinal chemistry·2025
Same author

Towards improved anticancer properties of the G-quadruplex-forming T40231 aptamer through increased flexibility of nucleoside residues.

Scientific reports·2025
Same author

A community child and adolescent psychiatry fellowship for nurse practitioners.

Archives of psychiatric nursing·2025
Same author

Thermodynamic control of mismatch discrimination for extensive splicing regulation of PKM pre-mRNA.

RNA (New York, N.Y.)·2025
Same author

Switching off cancer - An overview of G-quadruplex and i-motif functional role in oncogene expression.

Bioorganic & medicinal chemistry letters·2024
Same author

Revisiting the role of behavior-mediated structuring in the survival of populations in hostile environments.

Communications biology·2024

Video Experimental Relacionado

Updated: Sep 10, 2025

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

9.6K

Nucleótidos modificados en oligonucleótidos antisenso bifuncionales: explorando su potencial anticancerígeno

Natalia Bartyś1, Anna Pasternak1, Jolanta Lisowiec-Wąchnicka1

  • 1Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland.

ChemMedChem
|August 21, 2025
PubMed
Resumen

Los oligonucleótidos antisenso bifuncionales (BASOs) con modificaciones químicas específicas, como el β-L-ARN y el ácido nucleico desbloqueado, mejoran las propiedades anticancerígenas mediante la regulación del empalme genético y la reducción de la proliferación y la motilidad de las células cancerosas.

Palabras clave:
empalme alternativoOligonucleótidos antisenso bifuncionaleslínea de células cancerosasnucleótidos modificadosReglamento de empalme

Más Videos Relacionados

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication
05:33

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Published on: July 5, 2024

792
Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
15:22

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

17.2K

Videos de Experimentos Relacionados

Last Updated: Sep 10, 2025

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

9.6K
Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication
05:33

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Published on: July 5, 2024

792
Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
15:22

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

17.2K

Área de la Ciencia:

  • Biología molecular
  • La bioquímica
  • Investigación del cáncer

Sus antecedentes:

  • Los oligonucleótidos antisenso bifuncionales (BASOs) son herramientas de conmutación de empalme dirigidas al ARN pre-mensajero.
  • El empalme alternativo del gen piruvato quinasa M1/2 es crucial en el desarrollo del cáncer.
  • La comprensión de las modificaciones de nucleótidos puede mejorar la eficacia terapéutica de BASO.

Objetivo del estudio:

  • Investigar el impacto de los nucleótidos modificados en el empalme alternativo del gen M1/2 de la piruvatocinasa.
  • Evaluar el potencial anticancerígeno de los BASO modificados en las células cancerosas.
  • Identificar las modificaciones químicas que mejoran las propiedades terapéuticas de BASO.

Principales métodos:

  • Síntesis y aplicación de BASO modificados de diversas maneras.
  • Medición en tiempo real de la proliferación y la motilidad de las células cancerosas.
  • Análisis de la regulación alternativa del empalme por las BASO.

Principales resultados:

  • Las modificaciones químicas específicas mejoraron significativamente las propiedades terapéuticas de BASO.
  • Los BASO modificados redujeron la proliferación de células cancerosas, indujeron la muerte celular y disminuyeron la motilidad.
  • Las modificaciones del β-L-ARN y del ácido nucleico desbloqueado mostraron potentes efectos reguladores de empalme y anticancerígenos.

Conclusiones:

  • Las modificaciones químicas, particularmente el β-L-ARN y el ácido nucleico desbloqueado, mejoran la eficacia de BASO en la regulación del empalme y el tratamiento del cáncer.
  • Estas modificaciones ofrecen una estrategia prometedora para desarrollar nuevos tratamientos contra el cáncer basados en oligonucleótidos.
  • El estudio propone modificaciones eficientes para los oligonucleótidos que se unen a las proteínas para mejorar los resultados terapéuticos.