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MarK, una quinasa de Novosphingobium aromaticivorans requerida para el catabolismo de múltiples monómeros aromáticos

Benjamin W Hall¹, Jeanette C Neri¹, Craig A Bingman²

¹Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison, Wisconsin, USA.

The Journal of biological chemistry

|August 21, 2025

Resumen

Los investigadores diseñaron Novosphingobium aromaticivorans para metabolizar la acetovanillona, un compuesto aromático derivado de la lignina. Una sola mutación de aminoácido en Saro_1862 (MarK) permitió el crecimiento en acetovanillona al aumentar su tasa de fosforilación.

Área de la Ciencia:

Metabolismo microbiano y ingeniería enzimática
Biotecnología y recursos renovables

Sus antecedentes:

Los compuestos aromáticos son cruciales para la industria, pero se obtienen principalmente del petróleo.
La lignina, un polímero vegetal renovable, es una fuente abundante de compuestos aromáticos.
Novosphingobium aromaticivorans puede metabolizar algunos derivados de la lignina pero no la acetovanillona.

Objetivo del estudio:

Para permitir que Novosphingobium aromaticivorans utilice la acetovanillona como única fuente de carbono.
Identificar la base genética para el metabolismo de la acetovanillona en N. aromaticivorans.
Caracterizar la enzima responsable de la fosforilación de la acetovanillona.

Principales métodos:

Evolución adaptativa en el laboratorio de N. aromaticivorans.
Análisis genético para identificar las mutaciones que confieren la utilización de la acetovanillona.
Ensayos bioquímicos y cristalografía de proteínas para caracterizar la actividad y estructura de las enzimas.

Principales resultados:

Se identificó una sola sustitución de aminoácidos (E16K) en Saro_ 1862 como esencial para el crecimiento con acetovanillona.
La variante MarK E16K exhibe un aumento de la tasa de fosforilación de la acetovanilona dependiente del ATP de aproximadamente 1600 veces.
El MarK E16K recombinante fosforila compuestos aromáticos múltiples, definiendo la actividad catalítica del dominio UPF0261.

Conclusiones:

La enzima MarK (Saro_1862) es una nueva quinasa aromática múltiple.
La sustitución de E16K aumenta la actividad de MarK al alterar su sitio de unión al ATP.
Es probable que la vía identificada para el metabolismo aromático se conserve en toda la filogenia bacteriana.

Palabras clave:

Novosfingobio y sus derivados Acetovanillona y sus derivados Metabolismo aromático metabolismo bacteriano la enzima estructura de la enzima La kinasa degradación de la lignina Fosforilación

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