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Updated: Sep 10, 2025
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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
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Ciclases de terpenos no canónicos para la biosíntesis de diterpenoides que regulan la formación de clamidosporos en
Min-Jie Yang1, De-Sen Li1, Hua-Qin Deng1
1State Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, P. R. China.
Nature communications
|August 21, 2025
Resumen
Los investigadores descubrieron nuevas ciclas de terpenos (TriDTC) en los hongos Trichoderma. Estas enzimas crean diterpenoides complejos, cruciales para la supervivencia de los hongos y las aplicaciones de biocontrol.
Área de la Ciencia:
- La bioquímica
- Micología
- Química de los productos naturales
Sus antecedentes:
- Los hongos Trichoderma producen diterpenoides complejos (harzianes, trichodermaninas) con biosíntesis y función desconocidas.
- Las ciclases de terpeno son enzimas clave en la formación del esqueleto terpenoide.
- Comprender estas vías es vital para el control biológico y el descubrimiento de fármacos.
Objetivo del estudio:
- Identificar y caracterizar las nuevas ciclas de terpenos en Trichoderma.
- Aclarar el mecanismo y el origen evolutivo de estas enzimas.
- Investigar las funciones biológicas de los diterpenoides producidos.
Principales métodos:
- Expresión heteróloga de los genes candidatos.
- Deleción de genes y ensayos enzimáticos in vitro.
- Análisis filogenético y estudios funcionales.
Principales resultados:
- Identificó TriDTCs, una nueva familia de terpenos cicladas en Trichoderma.
- Se ha demostrado que los TriDTC ciclizan el difosfato de geranilgeranilo (GGPP) en harzianol I y wickerol A.
- Los TriDTC revelados poseen mecanismos catalíticos únicos y una historia evolutiva.
- Los TriDTC están vinculados a la formación de propágulos fúngicos resistentes.
Conclusiones:
- Los TriDTC representan una nueva clase de ciclas de terpenos con características únicas.
- Estas enzimas son cruciales para las estrategias de supervivencia de Trichoderma.
- Los hallazgos ofrecen potencial para mejorar la eficacia del biocontrol y explorar la farmacología de los diterpenoides.
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