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La perplejidad de la secuencia de ADN revela patrones evolutivamente conservados en regiones cis-reguladoras a través

Aruna Sesha Chandrika Gummadi¹, Venkata Rajesh Yella²

¹Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, 522302, India.

Biochemical genetics

|August 21, 2025

Resumen

Introducimos la perplejidad de la secuencia de ADN, una métrica teórica de la información, para identificar regiones cis-reguladoras entre especies. La menor perplejidad en estas regiones sugiere patrones conservados y evolutivamente estables en la regulación del ADN.

Área de la Ciencia:

La genómica
La bioinformática
Biología computacional

Sus antecedentes:

Comprender las regiones cis-reguladoras es crucial para descifrar los mecanismos fisiológicos y patológicos.
Las firmas regulatorias existentes, como los motivos y los potenciadores, muestran variaciones específicas de la especie, lo que dificulta las comparaciones entre especies.
Existe la necesidad de un método universal e independiente del motivo para caracterizar el ADN regulador.

Objetivo del estudio:

Introducir y validar la perplejidad de la secuencia de ADN como una nueva métrica para caracterizar las regiones cis-reguladoras.
Evaluar la conservación evolutiva de los patrones de perplejidad de secuencias en el ADN regulador.
Explorar la relación entre la perplejidad de la secuencia y la actividad transcripcional.

Principales métodos:

Utilizó la teoría de la información y el procesamiento del lenguaje natural para definir la perplejidad de la secuencia de ADN.
Se analizaron las regiones de inicio de transcripción y traducción en 1180 especies diversas.
Valores de perplejidad correlacionados con la fuerza del promotor utilizando conjuntos de datos de levadura.

Principales resultados:

Las regiones cis-reguladoras mostraron consistentemente una menor perplejidad de la secuencia de ADN que las regiones flanqueantes en todas las especies analizadas.
Este patrón de baja complejidad se conservó evolutivamente, independientemente de la clasificación taxonómica.
Los datos de levadura revelaron una correlación inversa entre la perplejidad y la fuerza del promotor, lo que indica una mayor transcripción con menor complejidad.

Conclusiones:

La perplejidad de la secuencia de ADN es una métrica efectiva e independiente del motivo para identificar el ADN cis-regulador conservado.
La perplejidad ofrece información sobre los principios generalizables de la arquitectura cis-regulatoria del ADN.
Este enfoque basado en la abstracción puede complementar los métodos basados en motivos para aplicaciones genómicas predictivas.

Palabras clave:

Región cis reguladora Perplejidad de la secuencia de ADN Promotor del proyecto Entropía de Shannon

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