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El retrómero regula la macro y micro autofagia a través de distintas proteasas vacuolares en el hongo blasta del

Dingyang Zhang¹, Jiexiong Hu¹, Yonghe Hong^1,2

¹State Key Laboratory of Agricultural and Forestry Biosecurity & Key Lab of Biopesticide and Chemical Biology, Ministry of Education, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|August 22, 2025

Resumen

El complejo de retrómeros es esencial para la autofagia en Magnaporthe oryzae, asegurando que las proteasas vacuolares se entreguen para la degradación de la carga. Esta regulación afecta el desarrollo y la patogenicidad de los hongos.

Área de la Ciencia:

Biología molecular
Biología celular
Micología

Sus antecedentes:

El vacío degrada la carga celular a través de la endocitosis y la autofagia.
El complejo de retrómeros media la recuperación de carga de los endosomas, evitando la degradación prematura.
El papel de los retrómeros en la regulación de las proteasas vacuolares durante la autofagia no se comprende bien.

Objetivo del estudio:

Investigar la función del complejo retrómero en la regulación del sistema proteolítico vacuolar durante la autofagia en Magnaporthe oryzae.
Determinar si el retrómero influye en la liberación y actividad de las proteasas vacuolares esenciales para la degradación del sustrato autofágico.

Principales métodos:

Se utilizaron mutantes de deleción genética de los componentes de los retrómeros (por ejemplo, MoVps35) y las proteasas vacuolares (MoPrb1, MoPep4) en M. oryzae.
Se empleó microscopía de fluorescencia para rastrear la localización de las proteasas vacuolares (por ejemplo, MoPrb1-GFP).
Se evaluó el flujo de autofagia a través de la mutación de los residuos catalíticos de MoPrb1 y se observó el desarrollo fúngico y la patogenicidad.

Principales resultados:

Los componentes del complejo de retrómero, incluido el MoVps35, son necesarios para la administración de proteasas vacuolares (MoPrb1) al lumen de la vacuola.
La eliminación de los componentes retrómeros o MoPrb1 afecta el flujo de autofagia, la conidiación y la infección de las plantas.
MoVps35 interactúa con MoPep4, y la pérdida de MoPep4 afecta a la microautofagia (pexofagia).

Conclusiones:

El complejo retrómero juega un papel crítico en la regulación tanto de la macro como de la micro autofagia en M. oryzae.
El retrómero asegura la entrega de proteasas vacuolares esenciales, como MoPrb1 y MoPep4, para una degradación eficiente de la carga.
Esta vía es vital para el desarrollo fúngico, la patogenicidad y la homeostasis celular en general.

Palabras clave:

Magnaporthe oryzae, también conocido como oryzae.Macro-autofagia Pexofagia complejo de retrómeros Las proteasas vacuolares

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