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El locus complejo de REDUCIDA COMPLEJIDAD contribuye a la diversificación de la forma de las hojas en Brassica rapa

Pan Li^1,2,3,4, Hongjia Xin^1,2,3,4, Jing Li^1,2,3,4,5

¹State Key Laboratory of Vegetable Biobreeding, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China.

Journal of experimental botany

|August 23, 2025

Resumen

Los investigadores identificaron BrRCO como un gen clave que regula la forma de las hojas en la col china (Brassica rapa). Las variaciones en su región promotora impulsan la diferenciación de las hojas, ofreciendo ideas para la mejora de los cultivos.

Área de la Ciencia:

Genética de las plantas
Biología del desarrollo
Ciencias agrícolas

Sus antecedentes:

La forma de la hoja es crucial para el valor económico y el desarrollo de la col china (Brassica rapa).
Los mecanismos genéticos que controlan la forma de la hoja en Brassica siguen siendo en gran medida desconocidos.

Objetivo del estudio:

Para aclarar la base molecular de la determinación de la forma de la hoja en la col china.
Para identificar el gen responsable de la formación de hojas lobuladas.

Principales métodos:

La clonación basada en el mapa para identificar el locus del rasgo cuantitativo (QTL) qBrLLA10.
Análisis de la secuencia de alelos parentales y del gen BrRCO.
La sobreexpresión ectópica en Arabidopsis y el silenciamiento genético inducido por virus (VIGS) en B. rapa.
Análisis sintético en las adhesiones de Brassica rapa.

Principales resultados:

Identificó BrRCO, un factor de transcripción HD-Zip, como el gen causal para la formación de hojas lobuladas.
Las variaciones de la región promotora en BrRCO explican las diferencias de forma de la hoja entre las líneas progenitoras.
BrRCO regula positivamente la formación del lóbulo de la hoja; su regulación a la baja bloquea el desarrollo del lóbulo.
Las regiones promotoras altamente variables y las regiones de codificación conservadas de BrRCO sugieren que la evolución cis-regulatoria impulsa la diversificación funcional.

Conclusiones:

BrRCO es un regulador positivo de la formación de los lóbulos de las hojas en Brassica rapa.
Las modificaciones cis-reguladoras en el promotor de BrRCO son responsables de la variación funcional y la diversidad de la forma de la hoja.
Los hallazgos proporcionan información para mejorar la forma de la hoja en la col china y otras especies de Brassica.

Palabras clave:

Las plantas de la familia Brassicaceae Las RCO Cantidad de tiempo Variación cis-regulatoria duplicación de genes el lóbulo de la hoja

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