Video Experimental Relacionado

Updated: Sep 10, 2025

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice

Published on: June 8, 2022

CircMTND5 participa en la lesión tubulointersticial en la nefritis por lupus al unirse con la proteína A1CF

Cong Ma¹, Junjun Luan¹, Congcong Jiao¹

¹Department of Nephrology, Shengjing Hospital of China Medical University, Shenyang, China.

International immunopharmacology

|August 24, 2025

Resumen

El ARN circular MTND5 (circMTND5) puede aliviar la fibrosis tubulointersticial por lupus nefritis (LN) al unirse a la proteína de unión al ARN A1CF. Esta interacción ayuda a regular los marcadores fibróticos en la progresión de la LN.

Área de la Ciencia:

Nefrología
Biología molecular
Inmunología

Sus antecedentes:

El lupus nefritis (LN) es una complicación común del lupus eritematoso sistémico.
El ARN circular MTND5 (circMTND5) está implicado en la patogénesis de la LN al interactuar con miR6812.
El papel de las proteínas de unión al ARN (RBPs) que interactúan con circMTND5 en la LN sigue sin estar claro.

Objetivo del estudio:

Investigar la interacción entre circMTND5 y RBPs en la progresión de la LN.
Aclarar los mecanismos subyacentes de circMTND5 en la patogénesis de la LN.
Determinar si el circMTND5 influye en la fibrosis tubulointersticial en el LN.

Principales métodos:

Para analizar la expresión de proteínas y circRNA en muestras de riñón de pacientes con LN y ratones, se utilizaron inmunostación, Western blot y qPCR.
Los ensayos de inmunoprecipitación de ARN (RIP) confirmaron la unión de circMTND5 a A1CF en las células renales humanas.
Los experimentos de reducción y sobreexpresión en células renales humanas modularon los niveles circMTND5 para evaluar su impacto funcional.

Principales resultados:

La expresión de A1CF disminuyó en los riñones LN y se localizó en las células epiteliales tubulares.
La eliminación de circMTND5 en las células renales condujo a una disminución de la E-caderina y a un aumento de α-SMA, TGF-β y fibronectina.
La sobreexpresión de circMTND5 revirtió los cambios fibróticos inducidos por TGF-β.

Conclusiones:

circMTND5 se une a la proteína de unión al ARN A1CF.
circMTND5 puede aliviar la fibrosis tubulointersticial en la nefritis por lupus.
La interacción circMTND5-A1CF es un mecanismo potencial para controlar la progresión de la LN.

Palabras clave:

A1CF (en inglés)Nefritis por lupus Fibrosis tubular y intersticial Circuito MTND5

Más Videos Relacionados

Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection

Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection

Published on: November 10, 2021

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Published on: November 4, 2016

Videos de Conceptos Relacionados

Nephrotic Syndrome I : Introduction

Nephrotic Syndrome I : Introduction

Nephrotic Syndrome is a chronic kidney disorder defined by clinical findings such as severe proteinuria, hypoalbuminemia, hyperlipidemia, and edema. These symptoms result from damage to the glomeruli, the kidney’s filtering units, increasing their permeability to proteins.Definition and Meaning:Proteinuria, defined as the loss of more than 3.5 grams of protein per day in adults, is a crucial feature of nephrotic syndrome. This condition is often accompanied by edema, the accumulation of...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

Acute Kidney Injury II: Pathophysiology

Acute Kidney Injury II: Pathophysiology

Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...

Type IV Collagen of Basal Lamina

Type IV Collagen of Basal Lamina

Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can...

Renal Corpuscle

Renal Corpuscle

The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...