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Cómo afecta el micro muestreo a los estudios farmacocinéticos y toxicocinéticos: micro muestreo volumétrico
Michele Protti1, Roberto Mandrioli2, Hugo M Santos3,4,5
1Research group of Pharmaco-Toxicological Analysis (PTA Lab), Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum - University of Bologna, Bologna, Italy.
Bioanalysis
|August 25, 2025
Resumen
El micro muestreo, utilizando muestras biológicas diminutas, ofrece ventajas para los estudios farmacocinéticos y toxicocinéticos. El microsamplamiento absorbente volumétrico (VAMS, por sus siglas en inglés) es una tecnología clave para el análisis cuantitativo fiable en estos estudios.
Área de la Ciencia:
- Farmacología y Toxicología
- Química analítica
- Ciencias Biomédicas
Sus antecedentes:
- El microsampling utiliza muestras biológicas mínimas, ofreciendo ventajas sobre las extracciones de sangre tradicionales.
- Los beneficios incluyen una mayor estabilidad del analito, una logística simplificada y un consumo reducido de material/animal.
- El micro muestreo absorbente volumétrico (VAMS) es una técnica de micro muestreo líder debido a su precisión de volumen.
Objetivo del estudio:
- Revisar los estudios farmacocinéticos y toxicocinéticos realizados con VAMS.
- Para resaltar la idoneidad de VAMS para el análisis cuantitativo.
- Presentar métodos analíticos compatibles con el VAMS para estos estudios.
Principales métodos:
- Revisión de la literatura de las aplicaciones de VAMS en estudios farmacocinéticos y toxicocinéticos.
- Análisis de las ventajas del VAMS sobre los métodos de muestreo convencionales.
- Compilación de técnicas analíticas de apoyo a la aplicación del VAMS.
Principales resultados:
- El VAMS demuestra un alto rendimiento y una fiabilidad comparables a los métodos tradicionales.
- VAMS ofrece mejoras significativas en la estabilidad y el manejo de muestras.
- Existen numerosos métodos analíticos para el análisis cuantitativo basado en VAMS.
Conclusiones:
- VAMS es una técnica de micro muestreo muy eficaz y ventajosa para la investigación farmacocinética y toxicocinética.
- Su precisión y la disponibilidad de métodos analíticos apoyan su adopción generalizada.
- VAMS representa un avance significativo en el análisis de muestras biológicas para el desarrollo de fármacos y la toxicología.
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