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El pequeño pangenoma de Candida parapsilosis refleja una baja diversidad intraspecífica en general

Adam P Ryan¹, Sean Bergin¹, Jillian Scully¹

¹School of Biomolecular and Biomedical Sciences, Conway Institute, University College Dublin, Dublin, Ireland.

|August 25, 2025

Resumen

Este estudio revela que la parapsilosis de Candida tiene una baja diversidad genética, con un pequeño pangénoma y variaciones en el contenido genético que afectan el metabolismo del nitrógeno. Una mayor vigilancia genómica puede descubrir más diversidad en este patógeno fúngico de alta prioridad.

Área de la Ciencia:

Microbiología
La genómica
Micología médica

Sus antecedentes:

La Candida parapsilosis es una levadura oportunista que causa infecciones graves en personas inmunocomprometidas.
Estudios anteriores indicaron una baja diversidad genética en C. parapsilosis basada en polimorfismos de un solo nucleótido (SNP).
Comprender la variación del contenido genético es crucial para este patógeno de alta prioridad de la OMS.

Objetivo del estudio:

Definir el pangénoma de 372 aislamientos de Candida parapsilosis para evaluar la variación del contenido genético.
Identificar los genes que contribuyen a la adaptabilidad y la virulencia de la especie.
Investigar la diversidad genética más allá del análisis SNP.

Principales métodos:

Análisis del pangénoma de los aislados de 372 C. parapsilosis.
Edición de genes CRISPR-Cas9 para estudiar la función genética.
Genómica comparativa para identificar los genes centrales y los accesorios.

Principales resultados:

El pangénoma de C. parapsilosis comprende 5.859 genes, con 48 genes accesorios que no están en la cepa de referencia.
Cuatro genes, incluido el DAL4, mostraron truncamientos en algunas cepas, afectando la utilización de la allantoína.
El genoma accesorio está enriquecido con genes relacionados con el transporte y las fusiones de genes.
Dos cepas muy divergentes exhibieron contenido genético similar a pesar de la distancia filogenética.

Conclusiones:

La parapsilosis por Candida exhibe una variación limitada del contenido genético a pesar de su importancia clínica.
Las variaciones en el contenido de genes, particularmente en los genes del metabolismo del nitrógeno como DAL4, pueden influir en la virulencia.
Se necesita una mayor vigilancia genómica para explorar toda la diversidad de C. parapsilosis.

Palabras clave:

Cándida La evolución análisis del genoma Las levaduras

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