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Metabolismo compartido entre una especie bacteriana y fúngica que reside en el intestino humano

Haley Gause¹, Alexander D Johnson^1,2

¹TETRAD Graduate Program, Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158.

Proceedings of the National Academy of Sciences of the United States of America

|August 25, 2025

Resumen

Las especies de hongos Candida albicans y la bacteria Enterococcus faecalis participan en un ciclo metabólico dentro del intestino. La desintoxicación cruzada de citrato y formato por C. albicans mejora el crecimiento de ambos microbios.

Área de la Ciencia:

Microbiología
Ecología microbiana
Biología de sistemas

Sus antecedentes:

Candida albicans y Enterococcus faecalis son miembros clave del microbioma intestinal humano.
Comprender las interacciones entre especies es crucial para descifrar la dinámica del ecosistema intestinal.

Objetivo del estudio:

Investigar las respuestas de transcripción y la interacción metabólica entre C. albicans y E. faecalis durante el cultivo conjunto.
Para aclarar los mecanismos que impulsan su coexistencia en el entorno intestinal.

Principales métodos:

Se empleó la secuenciación doble de ARN para perfilar la expresión génica de ambas especies en monocultivo y cocultivo.
Los experimentos se llevaron a cabo in vitro y en un modelo de intestino de ratón gnotobiótico.
El análisis funcional confirmó la alimentación cruzada de citrato y el papel de desintoxicación del formato.

Principales resultados:

El cocultivo indujo cambios transcripcionales significativos tanto en C. albicans como en E. faecalis.
Los genes del metabolismo del citrato fueron regulados al alza, con C. albicans produciendo citrato y E. faecalis utilizándolo, mejorando el crecimiento de E. faecalis.
C. albicans regula los genes de formato deshidrogenasa, lo que permite la desintoxicación del formato tóxico producido por E. faecalis, lo que confiere una ventaja de crecimiento.

Conclusiones:

Un ciclo metabólico que incluye alimentación cruzada de citrato y desintoxicación de formato facilita el co-crecimiento de C. albicans y E. faecalis.
Estos hallazgos resaltan la importancia de las interacciones metabólicas en la estructuración del microbioma intestinal.
El estudio revela una nueva simbiosis metabólica entre especies en el intestino.

Palabras clave:

Cándida albicans Enterococcus faecalis y sus derivados Secuencia de ARN El microbioma intestinal

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