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Un transcriptoma de latencia directo basado en ARN-seq del EBV ofrece información sobre la biogénesis de los

Aaron Mamane-Logsdon¹, Isabelle Zane¹, June See Chong¹

¹Section of Virology, Department of Infectious Disease, Imperial College London, London SW7 2AZ, UK.

The Journal of general virology

|August 26, 2025

Resumen

Los transcriptomas del virus de Epstein-Barr (EBV) fueron analizados mediante secuenciación de nanoporos. Este estudio revela nuevos patrones de empalme y elementos reguladores dentro de las transcripciones de latencia III del EBV, mejorando la investigación futura del EBV.

Área de la Ciencia:

Virología
Biología molecular
La genómica

Sus antecedentes:

El virus de Epstein-Barr (EBV) establece la persistencia de las células B durante toda la vida, formando líneas celulares linfoblásticas (LCL) in vitro.
Las transcripciones de latencia III del EBV codifican los antígenos nucleares del EBV (EBNA), las proteínas de membrana de latencia (LMP) y los ARN no codificantes (BART), con un extenso splicing alternativo.
La comprensión de la transcripción del EBV es crucial para descifrar la persistencia viral y la patogénesis.

Objetivo del estudio:

Caracterizar el transcriptoma completo del EBV en LCL utilizando secuenciación directa de ARN de larga lectura.
Identificar nuevos eventos de empalme, sitios de poliadenilación y límites de transcripción en la latencia del EBV III.
Desarrollar una anotación EBV mejorada para futuras investigaciones.

Principales métodos:

Generación de EBV-BAC con código de barras en LCL.
Secuenciación directa de ARN por nanoporo de larga lectura de las transcripciones de EBV.
Desarrollo de una tubería bioinformática para el mapeo y análisis preciso de transcripciones.

Principales resultados:

El empalme a través de la repetición interna 1 (IR1) incluye en gran medida todos los exones W, con transcripciones derivadas de Wp que favorecen a EBNA-LP.
Identificación de una variante de exón W2 corto y un nuevo sitio de poliadenilación aguas arriba de EBNA2.
Descubrimiento de transcripciones que se extienden a través de los límites de los genes (EBNA-BART-LMP2) y nuevos exones.
El cassette de BAC interrumpió la integridad de las transcripciones de BART.

Conclusiones:

La secuenciación directa de ARN proporciona una visión completa de la transcripción de latencia III del EBV.
Los nuevos eventos de empalme y poliadenilación ofrecen nuevos mecanismos reguladores para la expresión génica del EBV.
La anotación actualizada del EBV facilita una caracterización más precisa de los transcriptomas virales.

Palabras clave:

El virus de Epstein y Barr Secuencia de ARN directo la latencia El transcriptoma

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