Video Experimental Relacionado

Updated: Sep 10, 2025

Effective Oral RNA Interference RNAi Administration to Adult Anopheles gambiae Mosquitoes

Effective Oral RNA Interference RNAi Administration to Adult Anopheles gambiae Mosquitoes

Published on: March 1, 2022

Papel del gen Fox en el desarrollo y la reproducción de la polilla espinosa

Anam Noreen Abbas¹, Mubashir Tariq¹, Faisal Munir¹

¹State Key Laboratory of Agricultural and Forestry Biosecurity, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou 350002, China; Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou 350002, China; Key Laboratory of Green Pest Control, Fujian Province University, Fuzhou 350002, China.

Journal of insect physiology

|August 26, 2025

Resumen

El gen de la caja de horquilla 1 (PxFox1) es crucial para el desarrollo y la reproducción de la polilla espalda de diamante. La eliminación de PxFox1 en Plutella xylostella afecta significativamente la producción de huevos, la supervivencia y causa anormalidades de desarrollo, lo que sugiere que es un objetivo de control de plagas.

Área de la Ciencia:

Biología molecular de los insectos
Genética del desarrollo
Entomología agrícola

Sus antecedentes:

Los factores de transcripción de la caja de bifurcación (Fox) regulan las funciones vitales de los insectos.
El papel de los genes de Fox en la polilla del diamante (Plutella xylostella), una plaga crucífera clave, sigue sin caracterizarse.
La comprensión de PxFox1 es esencial para el manejo de P. xylostella.

Objetivo del estudio:

Para caracterizar el gen PxFox1 en P. xylostella.
Investigar la función de PxFox1 en la reproducción y el desarrollo de los insectos.
Para explorar PxFox1 como un objetivo para el control genético de plagas.

Principales métodos:

Identificación y análisis genómico de PxFox1 en P. xylostella.
Análisis de la expresión génica en diferentes etapas de desarrollo y tejidos.
La edición del gen CRISPR / Cas9 para crear mutantes knockout de PxFox1.
Análisis fenotípico de mutantes, incluida la producción reproductiva, el tiempo de desarrollo y la morfología.

Principales resultados:

PxFox1 se conserva en los lepidópteros y se expresa en gran medida en las hembras adultas y en el integumento.
Los mutantes knockout de PxFox1 mostraron un número significativamente reducido de huevos y tasas de eclosión.
Los mutantes exhibieron una duración larval prolongada, una fase pupal acortada, una vida adulta reducida y defectos morfológicos graves (por ejemplo, alas malformadas, segmentación irregular, tamaño corporal más pequeño).

Conclusiones:

PxFox1 es indispensable para el desarrollo normal, la supervivencia y la reproducción de P. xylostella.
Dirigirse a PxFox1 presenta una estrategia prometedora para desarrollar nuevos métodos genéticos de control de plagas.
Esta investigación proporciona información sobre la regulación genética de los insectos para el manejo sostenible de plagas.

Palabras clave:

CRISPR y Cas9 Factor de transcripción de las cabezas de tenedor Nocaut genético Manejo de plagas En el caso de las aves de corral, el número de ejemplares es de:

Más Videos Relacionados

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

Published on: October 6, 2016

Videos de Conceptos Relacionados

The Ratio of X Chromosome to Autosomes

The Ratio of X Chromosome to Autosomes

In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...

Background and Environment Affect Phenotype

Background and Environment Affect Phenotype

Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...

Position-effect Variegation

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.

Dosage Compensation

Dosage Compensation

In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...