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Updated: Sep 10, 2025
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A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
Published on: June 2, 2022
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La quinurenina promueve la calcificación endotelial inducida por fosfato a través de la transición endotelial a
Martina Molinaro1, Mario Cozzolino1, Paola Ciceri1
1Laboratory of Experimental Nephrology, Department of Health Sciences, University of Milan, 20142 Milan, Italy.
Toxins
|August 27, 2025
Resumen
Kynurenine agrava la calcificación vascular inducida por alto nivel de fosfato en la enfermedad renal terminal (ESRD) mediante la activación de la vía del receptor de hidrocarburos de arilo (AhR). Este hallazgo pone de relieve la
Área de la Ciencia:
- Nefrología
- Biología cardiovascular
- Biología celular
Sus antecedentes:
- Los pacientes con enfermedad renal en estadio terminal (ESRD, por sus siglas en inglés) enfrentan un alto riesgo cardiovascular debido a la acumulación de solutos.
- La calcificación vascular (VC) es una complicación importante en la ESRD, impulsada por la hiperfosfatemia y el entorno urémico.
- La quinurenina, una toxina urémica y un metabolito del triptófano, puede empeorar el VC.
Objetivo del estudio:
- Investigar si la cinurenina amplifica la calcificación inducida por el alto contenido de fosfato (Pi) en las células endoteliales aórticas humanas (HAEC).
Principales métodos:
- HAEC fueron tratados con Pi y kynurenine durante siete días.
- Se evaluó la deposición de calcio, la transición endotelial a mesenquimal (EndMT) y la diferenciación osteoblástica.
- Investigó el papel de la vía del receptor de hidrocarburos de arilo (AhR).
Principales resultados:
- Kynurenine aumentó la deposición de calcio inducida por Pi en un 36%.
- Kynurenine mejoró la EndMT y la diferenciación osteoblástica.
- Kynurenine activó la vía AhR; la inhibición de AhR redujo parcialmente estos efectos.
Conclusiones:
- La quinurenina potencia la calcificación vascular inducida por los fosfatos en la ERD.
- La vía del receptor de hidrocarburos de arilo (AhR) media los efectos pro-calcíficos de la cinurenina.
- La quinurenina contribuye a la disfunción endotelial en la ERD a través de la señalización AhR.
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