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Dasatinib inhibe la replicación del betacoronavirus en los macrófagos y atenúa los mediadores proinflamatorios a
Ana Carolina Santos Ricoy1, Marina Pimenta Braga1, Thaís Targino Ferreira Lacerda1
1Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Medical microbiology and immunology
|August 27, 2025
Resumen
Dasatinib (DASA) reduce la replicación y la inflamación del betacoronavirus al dirigirse a la señalización SRC en los macrófagos. Este estudio sugiere DASA como una terapia adyuvante potencial para las infecciones por coronavirus, impactando la entrada viral y las vías inflamatorias.
Área de la Ciencia:
- Virología
- Inmunología
- Farmacología
Sus antecedentes:
- Los betacoronavirus, como el SARS-CoV-2, causan enfermedades graves al desencadenar la inflamación en los macrófagos.
- Dasatinib (DASA), un inhibidor de la tirosina quinasa, ha mostrado efectos antiinflamatorios y antivirales más allá del tratamiento del cáncer.
Objetivo del estudio:
- Investigar Dasatinib (DASA) como un agente terapéutico potencial contra las infecciones por betacoronavirus.
- Para explorar el impacto de DASA en la replicación viral y las respuestas inflamatorias en los macrófagos.
Principales métodos:
- Modelo de infección in vitro utilizando células RAW 264.7 y el betacoronavirus MHV-3.
- Tratamiento con DASA antes y después de la infección.
- Medición de los títulos virales y de los mediadores proinflamatorios (IL-6, TNF, CXCL2).
- Análisis de la activación de la vía de señalización SRC-MAPK-NF-B.
Principales resultados:
- El tratamiento con DASA redujo significativamente los títulos virales del MHV-3 y los mediadores proinflamatorios.
- El tratamiento previo con DASA inhibió la entrada temprana del virus en los macrófagos.
- DASA modula la señalización de la tirosina quinasa SRC, suprimiendo la vía SRC-MAPK-NF-B.
- El tratamiento con DASA después de la infección redujo la liberación de mediadores inflamatorios.
Conclusiones:
- Dasatinib (DASA) muestra potencial como tratamiento adyuvante para las infecciones por betacoronavirus.
- DASA reduce efectivamente la replicación viral y la inflamación al dirigirse a las vías de señalización mediadas por SRC.
- La señalización de SRC presenta una estrategia viable para el control de las infecciones por coronavirus.
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