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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

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Riboswitches

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BioFuse: un interruptor de tiempo programable de la expresión génica

Cheng Huang¹, Jie Zhao¹, Zichun Tan¹

¹Institute of Biophysics, College of Life Sciences, Zhejiang University, Hangzhou, China.

Science advances

|August 27, 2025

Resumen

Este resumen es generado por máquina.

Desarrollamos BioFuse, un interruptor de tiempo de expresión génica programable con tiempos de reacción de horas a días. Esta herramienta de biología sintética controla con precisión la actividad génica para aplicaciones en ingeniería metabólica y biomedicina.

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Área de la Ciencia:

Biología sintética
Ingeniería genética
Biología molecular

Sus antecedentes:

Los sistemas de tiempo de expresión génica existentes tienen una sintonizabilidad temporal limitada debido a interacciones complejas con los reguladores.
El control preciso del tiempo de expresión génica es crucial para varias aplicaciones biotecnológicas.

Objetivo del estudio:

Desarrollar un interruptor de tiempo de expresión génica novedoso y programable con una amplia gama de sintonización temporal.
Para demostrar la utilidad de este temporizador en el control de las vías metabólicas y los procesos biológicos.

Principales métodos:

Diseñado BioFuse, un circuito sintético de genes que utiliza la edición secuencial de casetes de ADN por editores de bases de adenina (ABE).
Tiempos de reacción programados ajustando el número de casetes de ADN.
BioFuse aplicado para controlar los genes de biosíntesis de carotenoides en E. coli y un sistema de autólisis bacteriana.

Principales resultados:

BioFuse permite un tiempo de expresión génica programable que va desde horas hasta días.
Producción controlada de licopeno en E. coli, desacoplándola del crecimiento y aumentando el rendimiento.
Facilitar la liberación oportuna y eficiente de proteínas en un sistema de autólisis bacteriana.

Conclusiones:

BioFuse proporciona una herramienta versátil para el control temporal preciso y de amplio alcance de la expresión génica y las actividades metabólicas en las bacterias.
Esta tecnología tiene aplicaciones potenciales en biotecnología industrial y biomedicina.