Video Experimental Relacionado

Updated: Sep 10, 2025

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

BioFuse: un interruptor de tiempo programable de la expresión génica

Cheng Huang¹, Jie Zhao¹, Zichun Tan¹

¹Institute of Biophysics, College of Life Sciences, Zhejiang University, Hangzhou, China.

Science advances

|August 27, 2025

Resumen

Desarrollamos BioFuse, un interruptor de tiempo de expresión génica programable con tiempos de reacción de horas a días. Esta herramienta de biología sintética controla con precisión la actividad génica para aplicaciones en ingeniería metabólica y biomedicina.

Área de la Ciencia:

Biología sintética
Ingeniería genética
Biología molecular

Sus antecedentes:

Los sistemas de tiempo de expresión génica existentes tienen una sintonizabilidad temporal limitada debido a interacciones complejas con los reguladores.
El control preciso del tiempo de expresión génica es crucial para varias aplicaciones biotecnológicas.

Objetivo del estudio:

Desarrollar un interruptor de tiempo de expresión génica novedoso y programable con una amplia gama de sintonización temporal.
Para demostrar la utilidad de este temporizador en el control de las vías metabólicas y los procesos biológicos.

Principales métodos:

Diseñado BioFuse, un circuito sintético de genes que utiliza la edición secuencial de casetes de ADN por editores de bases de adenina (ABE).
Tiempos de reacción programados ajustando el número de casetes de ADN.
BioFuse aplicado para controlar los genes de biosíntesis de carotenoides en E. coli y un sistema de autólisis bacteriana.

Principales resultados:

BioFuse permite un tiempo de expresión génica programable que va desde horas hasta días.
Producción controlada de licopeno en E. coli, desacoplándola del crecimiento y aumentando el rendimiento.
Facilitar la liberación oportuna y eficiente de proteínas en un sistema de autólisis bacteriana.

Conclusiones:

BioFuse proporciona una herramienta versátil para el control temporal preciso y de amplio alcance de la expresión génica y las actividades metabólicas en las bacterias.
Esta tecnología tiene aplicaciones potenciales en biotecnología industrial y biomedicina.

Más Videos Relacionados

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Published on: May 7, 2018

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

Videos de Conceptos Relacionados

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

Transcriptional Regulation: Riboswitches

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...

Coordination of Gene Expression Processes in Bacteria

Coordination of Gene Expression Processes in Bacteria

The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...

Constitutive and Regulated Gene Expression

Constitutive and Regulated Gene Expression

Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...