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Updated: Sep 10, 2025
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Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model
Published on: July 12, 2018
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La motilidad de Vibrio cholerae está asociada con la transmisión entre animales
Ian W Campbell1, Ruchika Dehinwal1, Alexander A Morano1
1Division of Infectious Diseases at Brigham & Women's Hospital, and Department of Microbiology at Harvard Medical School, Boston, MA, USA.
Nature communications
|August 27, 2025
Resumen
Un gen de motilidad de Vibrio cholerae, motV, mejora la colonización intestinal y la enfermedad en ratones infantes. Esto conduce a una transmisión de cólera más eficiente entre animales, revelando nuevas ideas sobre la propagación de patógenos.
Área de la Ciencia:
- Microbiología
- Enfermedades infecciosas
- Patogénesis
Sus antecedentes:
- Los brotes de cólera son impulsados por el patógeno Vibrio cholerae.
- Comprender la transmisión de V. cholerae es crucial para controlar los brotes.
- Los modelos animales existentes no reflejan completamente la dinámica natural de transmisión entre animales.
Objetivo del estudio:
- Para identificar los factores de Vibrio cholerae que promueven la transmisión.
- Investigar el papel de la motilidad en la patogénesis y propagación de V. cholerae.
- Para vincular la colonización intra-animal y la enfermedad a la transmisión inter-animal.
Principales métodos:
- Se empleó una pantalla de transposones en ratones infantes para identificar los genes de V. cholerae que afectan la colonización y la transmisión.
- Un mutante de deleción motV (ΔmotV) se caracterizó por su motilidad, localización y capacidades de colonización.
- Se evaluó la eficiencia de transmisión del mutante ΔmotV y del tipo salvaje V. cholerae entre compañeros de camada.
Principales resultados:
- La inactivación del gen motV ligado a la motilidad aumentó significativamente la colonización intestinal de ratones infantes por V. cholerae.
- El mutante ΔmotV exhibió una mayor motilidad, una localización más temprana y más amplia en el intestino delgado y una mayor carga patógena.
- La eliminación de motV dio lugar a una transmisión más eficiente de V. cholerae entre los ratones infectados y los no infectados, incluso sin la toxina del cólera.
Conclusiones:
- El gen motV de V. cholerae juega un papel crítico en la regulación de la colonización intra-animal y la gravedad de la enfermedad.
- Los fenotipos asociados a la motilidad, más allá de la diarrea inducida por la toxina del cólera, contribuyen significativamente a la transmisión de V. cholerae.
- Este estudio establece un vínculo entre la colonización, la enfermedad y la propagación eficiente entre animales de V. cholerae.
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