Video Experimental Relacionado

Updated: Sep 10, 2025

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Published on: September 17, 2016

CRY1 La lisina 151 regula los ritmos circadianos a través de interacciones con las proteínas independientes de la

Jiawen Peng¹, Na Liu¹, Yixuan Ren¹

¹Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, College of Life Sciences, Hubei Normal University, Huangshi 435002, China.

International journal of molecular sciences

|August 28, 2025

Resumen

El estudio identifica a la lisina 151 (K151) en el criptocromo 1 (CRY1) como crucial para regular los ritmos circadianos. La modificación de K151 acorta el período circadiano, lo que sugiere un papel no degradativo en la función del reloj.

Área de la Ciencia:

* Biología molecular
* Cronología
* La bioquímica

Sus antecedentes:

* Los ritmos circadianos de los mamíferos están regulados por circuitos de retroalimentación transcripción-traducción.
* La proteína de reloj del núcleo criptocromo 1 (CRY1) es fundamental para estos ritmos.
* El papel preciso de los sitios de ubiquitinación CRY1 en la regulación del reloj no se comprende completamente.

Objetivo del estudio:

* Para investigar la función de sitios específicos de ubiquitinación en CRY1.
* Para aclarar los mecanismos no canónicos por los que CRY1 regula el período circadiano.

Principales métodos:

* Mutagénesis dirigida al sitio para crear mutantes CRY1-K151Q/R.
* Ensayos de rescate circadiano en células con deficiencia de Cry1/2.
* Ensayos de luciferasa para la represión transcripcional y ensayos de complementación de luciferasa para las interacciones proteicas.
* Análisis de la estabilidad de las proteínas y de la unión a la E3 ligasa.

Principales resultados:

* La mutación de lisina 151 (K151) a glutamina (Q) o arginina (R) acortó significativamente el período circadiano.
* Los mutantes K151Q/ R mostraron una reducción de la represión transcripcional y una interacción debilitada con BMAL1.
* Los mutantes mostraron una mayor unión a FBXL12 pero no a FBXL3, con una mayor estabilidad.
* La evidencia sugiere un mecanismo independiente de la ubiquitinación, excluyendo la degradación a través de FBXL3.

Conclusiones:

* El residuo CRY1 K151 es crítico para ajustar la periodicidad circadiana.
* Es probable que K151 funcione como un eje estructural que modula las interacciones de las proteínas, no a través de la rotación mediada por la ubiquitina.
* Los hallazgos redefinen la regulación post-traducional de CRY1 y sugieren nuevos objetivos terapéuticos para los trastornos circadianos.

Palabras clave:

reloj circadiano criptocromo 1 Interacciones con las proteínas Independiente de la ubiquitinación

Más Videos Relacionados

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

Published on: September 28, 2017

Videos de Conceptos Relacionados

Circadian Rhythms and Gene Regulation

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...

Regulated Protein Degradation

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...

Biological Clocks and Seasonal Responses

Biological Clocks and Seasonal Responses

The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

Covalently Linked Protein Regulators

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

Circadian rhythms are cyclic changes that are crucial in plasma drug concentrations. Various standard circadian parameters, including core body temperature, heart rate, and other cardiovascular factors, directly impact disease states and the therapeutic response to drug therapy.
The time of drug administration is an important factor to consider, as it can influence the toxic dose of a drug. For example, a study conducted by Prins et al. in 1997 examined the effects of the timing of...