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Integración de transcriptomas de longitud completa y segunda generación para elucidar la reprogramación
Xinlei Liu1, Ying Li1, Xinfeng Yang2
1Henan Key Laboratory of Funiu Mountain Insect Biology, China-UK International Joint Laboratory for Insect Biology of Henan Province, Nanyang Normal University, Nanyang 473061, China.
Insects
|August 28, 2025
Resumen
Este estudio revela cómo la infección por Autographa californica nucleopolyhedrovirus (AcNPV) reprograma el intestino medio de Antheraea pernyi. El AcNPV interrumpe el metabolismo del huésped y las respuestas inmunes al alterar la expresión génica y las interacciones proteicas.
Área de la Ciencia:
- * Biología molecular
- * Entomología
- * Virología
Sus antecedentes:
- * El Antheraea pernyi midgut es crucial para la defensa antiviral.
- * La complejidad transcripcional en este órgano sigue sin caracterizarse en gran medida.
- * La comprensión de las interacciones virales requiere un conocimiento detallado de las respuestas del huésped.
Objetivo del estudio:
- * Para crear un atlas de transcriptoma completo del intestino medio de A. pernyi.
- * Para identificar nuevos genes, isoformas, ARN largos no codificantes (ARNlnc) y factores de transcripción (TF).
- * Para investigar los cambios en la transcripción durante la infección por el virus nucleopolíedrico Autographa californica (AcNPV).
Principales métodos:
- * Técnicas integradas de PacBio Iso-Seq y RNA-seq para la secuenciación del transcriptoma.
- * Análisis diferencial de la expresión génica para identificar cambios después de la infección por AcNPV.
- * Análisis de enriquecimiento de vías y de redes de interacción proteína-proteína.
Principales resultados:
- * Se identificaron 1850 nuevos genes codificadores de proteínas, 17.736 nuevas isoformas, 1664 nuevos lncRNA y 858 TF.
- * Se detectaron 2471 eventos alternativos de empalme y 3070 sitios alternativos de poliadenilación.
- * Se ha revelado una expresión diferencial significativa de genes, isoformas, lncRNAs y TFs durante la infección por AcNPV, afectando el metabolismo, la síntesis de ADN, la autofagia y las vías de apoptosis.
Conclusiones:
- * La infección por AcNPV altera significativamente el paisaje transcripcional del huésped y las redes reguladoras en el intestino medio de A. pernyi.
- * El virus reprograma el metabolismo energético del huésped, los procesos biosintéticos y las respuestas inmunológicas.
- * Los hallazgos proporcionan una base para comprender las interacciones A. pernyi-virus y los mecanismos de defensa del huésped.
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