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Los remodeladores de cromatina asociados a RdDM en la soja: evolución y expresión inducida por el estrés de los genes

Paula Machado de Araújo¹, Arthur Gruber², Liliane Santana Oliveira²

¹Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes 28013-602, RJ, Brazil.

Plants (Basel, Switzerland)

|August 28, 2025

Resumen

Este estudio identifica nuevos genes CLASSY (CLSY) en la soja y analiza sus relaciones evolutivas. Estos reguladores epigenéticos muestran expresión modulada por el estrés, ofreciendo información sobre la soja

Área de la Ciencia:

La epigenética y la biología molecular
Genómica de las plantas
Biología evolutiva

Sus antecedentes:

La metilación dirigida por ARN (RdDM) es un mecanismo epigenético clave en las plantas, que involucra proteínas de remodelación de cromatina como CLASSY (CLSY).
El papel de la familia de genes CLSY en la soja, un cultivo vital, no se entiende bien.
Las investigaciones anteriores identificaron proteínas CLSY principalmente en la Arabidopsis thaliana.

Objetivo del estudio:

Identificar nuevos miembros de la familia CLSY en la soja mediante el uso de modelos de Markov ocultos de perfil (MMH de perfil).
Investigar las relaciones filogenéticas de los genes CLSY en diversos linajes de plantas.
Explorar los patrones de expresión de los genes CLSY en la soja bajo condiciones de estrés abiótico.

Principales métodos:

Se utilizaron HMM de perfil para la identificación del gen CLSY en la soja y otras especies vegetales.
Se realizaron análisis filogenéticos para comprender la evolución del gen CLSY.
Utilizó RT-qPCR para evaluar la expresión génica bajo estrés osmótico y salino durante la germinación.

Principales resultados:

Se han identificado dos nuevos candidatos de soja CLSY1-2 y un candidato DRD1.
Se detectaron por primera vez los genes CLSY y DRD1 en Aquilegia coerulea.
El análisis filogenético reveló dos grupos principales de CLSY, con la expansión de la familia de soja vinculada a eventos de duplicación genética.
CLSY y otros reguladores epigenéticos mostraron una expresión modulada por el estrés en las plántulas de soja.

Conclusiones:

Mejora la comprensión de la evolución y los mecanismos de expansión de la familia CLSY en las plantas, particularmente en la soja.
Proporciona nuevos conocimientos sobre el papel de los genes CLSY en la respuesta de la soja al estrés abiótico.
Destaca la importancia de la regulación epigenética en la adaptación de los cultivos a los desafíos ambientales.

Palabras clave:

CLSY1-4 (en inglés)Glicina máxima Metilación del ADN dirigida por ARN Regulación epigenética Filogénesis El perfil oculto de los modelos de Markov

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