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Conocimiento genómico sobre la complejidad de la enfermedad viral del fruto de la pasión

Florence Munguti¹, Evans N Nyaboga², Timothy Holton³

¹Kenya Plant Health Inspectorate Service, Nairobi, Kenya.

Microbiology spectrum

|August 29, 2025

Resumen

Suécia

Área de la Ciencia:

Virología vegetal
La genómica
Filogenética

Sus antecedentes:

Las enfermedades virales de la fruta de la pasión tienen un impacto significativo en la industria de la fruta de la pasión de Kenia.
Los síntomas de la enfermedad de la leña, incluido el endurecimiento de los frutos y la distorsión de las hojas, son frecuentes.
Este es el primer estudio exhaustivo de las enfermedades virales de la fruta de la pasión en Kenia.

Objetivo del estudio:

Realizar estudios exhaustivos sobre el virus en las regiones kenianas de cultivo de fruta de la pasión.
Caracterizar los genomas de los virus identificados y comprender su diversidad genética.
Proponer una nomenclatura neutral para los virus identificados e informar sobre las estrategias de gestión.

Principales métodos:

Encuestas de campo en los principales condados productores de fruta de la pasión en Kenia.
Secuenciación genómica de aislamientos virales, incluido el ensamblaje completo del genoma.
Análisis filogenéticos y de recombinación para determinar las relaciones e introducciones virales.
Estudios de correlación de síntomas para comprender los impactos de la coinfección.

Principales resultados:

Identificó el virus de la pasiflora de Uganda (UPV) y el virus de distorsión de la pasiflora de Asia oriental (EAPDV) como los principales patógenos.
Secuenciado 23 genomas completos de UPV y 2 genomas de EAPDV de Kenia.
Las cepas de UPV mostraron un 99% de identidad de nucleótidos con los aislados de Uganda; EAPDV mostró un 66% de identidad con UPV.
Se detectaron linajes filogenéticos distintos que sugieren múltiples introducciones y posibles coinfecciones sinérgicas.

Conclusiones:

Se propone cambiar el nombre de EAPDV a virus de distorsión de la pasiflora (PDV) y el de UPV a virus de la pasiflora (PV).
Destacó el papel de las coinfecciones en la exacerbación de la gravedad de la enfermedad.
Se recomienda una gestión estricta, una vigilancia reforzada y una certificación mejorada de los viveros para proteger a la industria.

Palabras clave:

Metagenomía Enfermedad de las frutas de la pasión Los virus

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