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Mapeo del paradigma evolutivo del virus de la diarrea bovina Npro asociado con diferentes organizaciones de
Xili Feng1,2, Zeyu Liu1, Xiaoting Ren1
1Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou, China.
Virulence
|August 29, 2025
Resumen
La evolución de la proteína no estructural (Npro) del virus de la diarrea bovina (BVDV) está determinada por el sesgo de nucleótidos y el uso de codones, lo que influye en la adaptación viral y la evasión inmune del huésped. Este estudio revela patrones evolutivos únicos más allá del genotipo simple.
Área de la Ciencia:
- Virología
- Biología molecular
- La genética evolutiva
Sus antecedentes:
- La proteína no estructural (Npro) del virus de la diarrea bovina (BVDV) es un factor de virulencia clave.
- Npro deteriora la inmunidad antiviral del huésped y mejora la replicación viral.
- La comprensión de las presiones selectivas en la evolución de BVDV Npro es crucial.
Objetivo del estudio:
- Investigar cómo las presiones selectivas influyen en los pares de nucleótidos, los codones sinónimos y el sesgo de codones dependientes del contexto (CDCB) en BVDV Npro.
- Explorar los mecanismos evolutivos que dan forma a la arquitectura de nucleótidos de BVDV Npro en todos los genotipos.
Principales métodos:
- Análisis de la composición de nucleótidos, frecuencias de dinucleótidos (CpG, UpA, UpG), uso de codones sinónimos y CDCB en las secuencias de BVDV Npro.
- Comparación de estos patrones en diferentes genotipos de VVDB.
Principales resultados:
- El genotipo 1 de BVDV muestra un sesgo de uso de nucleótidos más fuerte en Npro a pesar de tener más subgenotipos.
- Los dinucleótidos CpG y UpA se suprimen, mientras que el UpG se selecciona con frecuencia en todos los genotipos.
- Npro exhibe un pronunciado sesgo y variabilidad en el uso de pares de nucleótidos y CDCB debido a altas tasas de mutación y presiones selectivas.
Conclusiones:
- La arquitectura de los nucleótidos y el CDCB en BVDV Npro muestran patrones evolutivos únicos independientes del genotipo.
- La selección traslacional y la respuesta inmune del huésped determinan significativamente la composición del nucleótido Npro.
- El diverso repertorio de nucleótidos en Npro facilita la adaptación viral y la evasión del sistema inmunológico.
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