Video Experimental Relacionado
Updated: Sep 9, 2025
10:55
Rescue of Recombinant Newcastle Disease Virus from cDNA
Published on: October 11, 2013
19.5K
El virus de la enfermedad de Newcastle promueve la propagación de la infección a través de la lesión de las uniones
Xiaolong Lu1,2,3, Qiwen Zhou1, Mingzhu Wang1
1Key Laboratory of Avian Bioproducts Development, Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou, China.
PLoS pathogens
|August 29, 2025
Resumen
El virus de la enfermedad de Newcastle (NDV) causa enfermedades respiratorias al dañar las uniones estrechas, que son vitales para la integridad celular. Este daño viral facilita la replicación y propagación del NDV, empeorando el daño pulmonar en las aves de corral.
Área de la Ciencia:
- Virología
- Biología celular
- Patogénesis
Sus antecedentes:
- El virus de la enfermedad de Newcastle (VND) es un patógeno importante de las aves de corral que se propaga por las vías respiratorias.
- Los mecanismos exactos de propagación y patogénesis de la infección por NDV siguen siendo incompletamente entendidos.
Objetivo del estudio:
- Investigar el papel de la integridad de las uniones estrechas (TJ) en la replicación y la propagación del VDN.
- Aclarar los mecanismos moleculares por los que el NDV interrumpe la función de la tiroides y promueve la diseminación viral.
Principales métodos:
- Análisis de la expresión de la proteína TJ (OCLN, ZO-1) y la localización después de la infección por NDV.
- Investigar la participación de las proteínas citosqueléticas (vimentina, F-actina) y las vías de señalización (MLC/p-MLC).
- Utilizando técnicas de eliminación de genes para evaluar la importancia funcional de las proteínas TJ.
Principales resultados:
- La infección por NDV redujo significativamente los niveles de OCLN y ZO-1 a través de vías de degradación, comprometiendo la integridad de la tiroides.
- NDV indujo reorganizaciones de vimentina y F-actina, formando estructuras parecidas a jaulas que mejoraron la lesión de la tiroides y la replicación viral.
- La reorganización de la vimentina fue crítica para la redistribución de OCLN/ZO-1 y facilitó la propagación viral, la inflamación y el daño pulmonar, particularmente por las cepas virulentas de NDV.
Conclusiones:
- La lesión de las uniones cerradas inducida por NDV es un mecanismo clave para la replicación y propagación del virus.
- La alteración de la integridad de la tiroides mediada por vimentina es esencial para la patogénesis de la NDV y la exacerbación del daño pulmonar.
- Comprender estos mecanismos ofrece información sobre el tratamiento de las neumonías virales, incluidas las causadas por los coronavirus y los virus de la gripe.
Videos de Conceptos Relacionados
01:24Adherens Junctions
5.0K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
5.0K
01:29Tight Junctions
5.5K
Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
5.5K
02:30Metastasis
5.7K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
5.7K
01:35Cancer Cell Migration through Invadopodia
2.4K
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
2.4K
01:17Intracellular Signaling Affects Focal Adhesions
2.8K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.8K
01:10Intracellular Movement of Viruses and Bacteria
2.9K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.9K